Objectives To search the method of culturing human trabecular cells (HTC) on a filter support so as to provide a model to study the hydraulic conductivity of HTC in vivo. Methods The third passage of HTC was cultured on a nylon filter; after that we measured the rate of different irrigations through the filter with HTC [Lp, mu l/ (min.mm Hg.cm(2))]. Results HTC could continuously grow on the filters. The normal Lp was 10. 45 mu l/(min.mm Hg.cm(2)). Irrigated by the solution of epinephrine (EPI) or dexamethasone (DEX), Lp of HTC were higher than that in controls of the same cultural time, while after being exposed to DEX for a few days, Lp was significantly decreased. Conclusions (1) More information of hydraulic conductivity and effects of pharmacologic agents on HTC could be got from the dynamic filtery model; (2) EPI could improve the conductivity of HTC while DEX could have the same effect in early period.