机构：[a]Department of Physiology, School of Basic Medical Sciences, Beijing Medical University, Beijing (People's Rep. China),[b]Department of Orthopedics, The First University Hospital, Beijing Medical University, Beijing (People's Rep. China),[c]Department of Anesthesiology, Xuan-Wu Hospital, Beijing (People's Rep. China),麻醉手术科首都医科大学宣武医院[d]Department of Drug Dependence Research, Karolinska Institute, 104 O1 Stockholm (Sweden)
Transcutaneous nerve stimulation (TENS) treatment was given for 30 min to 37 patients divided into 3 groups of 10 patients and 1 group of 7 patients. Two groups received low-frequency (2 Hz) and the other 2 groups high-frequency (100 Hz) stimulation. A diagnostic lumbar cerebrospinal fluid (CSF) sample was obtained immediately before and after stimulation. The CSF samples were subjected to analysis of immunoreactive (ir) opioid peptides. Met-enkephalin-Arg-Phe (MEAP) from preproenkephalin and dynorphin A (Dyn A) from preprodynorphin, respectively. Low frequency TENS applied on the hand and the leg resulted in a marked increase (367%, P < 0.05) of ir-MEAP but not ir-Dyn A, whereas high-frequency (100 Hz) TENS produced a 49% increase in ir-Dyn A (P < 0.01) but not ir-MEAP. This is the first report in humans that 2 Hz and 100 Hz peripheral stimulation induces differential release of peptides from preproenkephalin and preprodynorphin, respectively.
基金：
Swedish Medical Research Council and by the National Natural Science Foundation of China and the National Institute of Drug Abuse, Rockville, MD, U.S.A.
第一作者机构：[a]Department of Physiology, School of Basic Medical Sciences, Beijing Medical University, Beijing (People's Rep. China),
通讯作者：
通讯机构：[*]Department of Drug Dependence Research, Karolinska Institute, P.O. Box 60500, 104 01 Stockholm, Sweden.
推荐引用方式(GB/T 7714)：
J.S. Han,X.H. Chen,S.L. Sun,et al.EFFECT OF LOW-FREQUENCY AND HIGH-FREQUENCY TENS ON MET-ENKEPHALIN-ARG-PHE AND DYNORPHIN-A IMMUNOREACTIVITY IN HUMAN LUMBAR CSF[J].PAIN.1991,47(3):295-298.doi:10.1016/0304-3959(91)90218-M.
APA：
J.S. Han,X.H. Chen,S.L. Sun,X.J. Xu,Y. Yuan...&L. Terenius.(1991).EFFECT OF LOW-FREQUENCY AND HIGH-FREQUENCY TENS ON MET-ENKEPHALIN-ARG-PHE AND DYNORPHIN-A IMMUNOREACTIVITY IN HUMAN LUMBAR CSF.PAIN,47,(3)
MLA：
J.S. Han,et al."EFFECT OF LOW-FREQUENCY AND HIGH-FREQUENCY TENS ON MET-ENKEPHALIN-ARG-PHE AND DYNORPHIN-A IMMUNOREACTIVITY IN HUMAN LUMBAR CSF".PAIN 47..3(1991):295-298
医学