机构：[1]Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China[2]Department of General Surgery, Xuanwu Hospital, Capital University of Medical Science, Beijing 100053, China普通外科首都医科大学宣武医院[3]Department of Laboratory Animal Science, Capital University of Medical Science, Beijing 100069, China
Objective:To investigate the nature of the restoration of cell polarity and bile excretion function in Sandwich-cultured hepatocytes.Methods:Freshly isolated hepatocytes from male Sprague-Dawley rats were cultured in a double layer collagen gel Sandwich configuration.Morphological changes were observed under a inverted microscope.The domain specific membrane associated protein DPP Ⅳ was tested by immunofluorescenee,and the bile excretion function was determined by using fluorescein diacetate.Hepatocytes cultured on a single layer of collagen gel were taken as control.Results:Adult rat hepatocytes cultured in a double layer collagen gel sandwich configuration regained its morphological and functional polarity and maintained polygonal morphology for at least 4 weeks.Immunofluorescence studies USing antibodies against DPP Ⅳ showed polarity restoration as early as 48 h.After cultured in the double layer collagen gel Sandwich configuration for 96 h the hepatocytes began to excrete bile;while hepatocytes cultured on a single layer collagen gel had no bile excretion.Conclusion:Hepatocytes cultured in a double layer collagen gel Sandwich configuration are able to regain their morphological and functional polarity givan certain conditions.Hepaotcyte culture is a useful tool for the study of polarity restoration.
基金：
the National Natural Science Foundation of China (No. 30571763)
Beijing Municipal Science & Technology Commission (2004B29)
语种：
外文
第一作者：
第一作者机构：[1]Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
通讯作者：
通讯机构：[1]Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
推荐引用方式(GB/T 7714)：
ZHANG Xian-jie,WANG Ying,SUN Jia-bang,et al.Restoration of cell polarity and bile excretion function of hepatocytes in sandwich-culture[J].JOURNAL OF MEDICAL COLLEGES OF PLA.2007,22(2):82-86.doi:10.1016/S1000-1948(07)60018-5.
APA：
ZHANG Xian-jie,WANG Ying,SUN Jia-bang,SONG Mao-min&QIAO Xin.(2007).Restoration of cell polarity and bile excretion function of hepatocytes in sandwich-culture.JOURNAL OF MEDICAL COLLEGES OF PLA,22,(2)
MLA：
ZHANG Xian-jie,et al."Restoration of cell polarity and bile excretion function of hepatocytes in sandwich-culture".JOURNAL OF MEDICAL COLLEGES OF PLA 22..2(2007):82-86
