机构：[1]Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[2]Department of Radiation Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[3]Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215006, China[4]Department of Pathology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China[5]Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[6]Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[7]Department of Pathology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[8]Department of Oncology, The First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310006, China[9]Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[10]Xi'an Tianlong Science and Technology Co., Ltd., Xi'an 710018, China[11]PREMED Key Laboratory for Precision Medicine, Soochow University, Suzhou 215021, China[12]Jiangsu Institute of Clinical Immunology, Suzhou 215006, China[13]Institute of Medical Biotechnology, Soochow University, Suzhou 215021, China
Squamous cell carcinoma (SCC) of pancreas is a rare histotype of pancreatic ductal carcinoma which is distinct from pancreatic adenocarcinoma (AC). Although there are standard treatments for pancreatic AC, no precise therapies exist for pancreatic SCC. Here, we screened 1033 cases of pancreatic cancer and identified 2 cases of pure SCC, which were pathologically diagnosed on the basis of finding definite intercellular bridges and/or focal keratin peal formation in the tumor cells. Immunohistochemistry assay confirmed the positive expression of CK5/6 and p63 in pancreatic SCC. To verify the genomic characteristics of pancreatic SCC, we employed in-solution hybrid capture targeting 137 cancer-related genes accompanied by high throughput sequencing (HTS) to compare the different genetic variants in SCC and AC of pancreas. We compared the genetic alterations of known biomarkers of pancreatic adenocarcinoma in different pancreatic cancer tissues, and identified nine mutated genes in SCC of pancreas: C7orf70, DNHD1, KPRP, MDM4, MUC6, OR51Q1, PTPRD, TCF4, TET2, and nine genes (ABCB1, CSF1R, CYP2C18, FBXW7, ITPA, KIAA0748, SOD2, SULT1A2, ZNF142) that are mutated in pancreatic AC. This study may have taken one step forward on the discovery of potential biomarkers for the targeted treatment of SCC of the pancreas.
基金：
This work was supported by grants from the National Natural Science Foundation of China (grant nos. 81472296, 81101867, 81602091, 81272542, 81200369, 81572992 and 81501970), the Six Major Talent Peak Project of Jiangsu Province (grant number 2015-WSN- 022), the China International Medical Foundation (grant no. CIMF-F-H001-057), the Special Foundation of Clinical Medicine of Jiangsu Provincial Bureau of Science and Technology (grant no. BL2014039), the Scientific Research Project of Jiangsu Provincial Bureau of Traditional Chinese Medicine (grant no. L213236), the Medical Scientific Research Project of Jiangsu Provincial Bureau of Health (grant no. Z201206), the Special Foundation of Wu Jieping Medical Foundation for Clinical Scientific Research (grant nos. 320.6753.1225 and 320.6750.12242), the Science and Education for Health Foundation of Suzhou for Youth (grant nos. kjxw2015003, SWKQ1003 and SWKQ1011), the Science and Technology Project Foundation of Suzhou (grant nos. SYS201112, SYSD2012137, SYSD201464 and SYS201335).
第一作者机构：[1]Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[11]PREMED Key Laboratory for Precision Medicine, Soochow University, Suzhou 215021, China[12]Jiangsu Institute of Clinical Immunology, Suzhou 215006, China[13]Institute of Medical Biotechnology, Soochow University, Suzhou 215021, China
推荐引用方式(GB/T 7714)：
Xu Meng-Dan,Liu Shu-Ling,Feng Yi-Zhong,et al.Genomic characteristics of pancreatic squamous cell carcinoma, an investigation by using high throughput sequencing after insolution hybrid capture[J].Oncotarget.2017,8(9):14620-14635.doi:10.18632/oncotarget.14678.
APA：
Xu, Meng-Dan,Liu, Shu-Ling,Feng, Yi-Zhong,Liu, Qiang,Shen, Meng...&Li, Wei.(2017).Genomic characteristics of pancreatic squamous cell carcinoma, an investigation by using high throughput sequencing after insolution hybrid capture.Oncotarget,8,(9)
MLA：
Xu, Meng-Dan,et al."Genomic characteristics of pancreatic squamous cell carcinoma, an investigation by using high throughput sequencing after insolution hybrid capture".Oncotarget 8..9(2017):14620-14635
医学