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Study on effect of α-synuclein enhanced survival rate of rat primary cultured neuron

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机构： [1]Department of Human Anatomy, School of Basic Medicine Sciences, Beihua University, Jilin 132013, P.R.China [2]College of Science, Jilin Institute of Chemical Technology, Jilin 132022 P. R. China [3]Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education Beijing 100053

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关键词： α-Synuclein Parkinson's Disease primary neuronal culture

摘要：

To investigate the effect of α-Synuclein(α-Syn) on the survival rate of brain neurons. We isolated neurons from the neocortex of newborn rats were cultured and added α-Syn into the culture medium to observe the effect of the protein on the primary cultured neurons. In addition, the effect of different concentrations of α-Syn on the neuron growth was studied. The mean survival rate of primary cultured neurons treated with α-Syn was significantly highter than that of control neurons at 1h, 2h and 4h. The effect of α-Syn on increasing the primary cultured survival rate wasn't change with the increasing of α-Syn concentration and was reversed by anti-α-Syn monoclonal antibody. Western blot and Immunofluorescence staining assay showed α-Syn can enter into the neuron. We indicate that α-Syn can increase the survival rate of primary cultured neuron and it's a new physiological function of α-Syn. © 2011 IEEE.

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第一作者机构： [1]Department of Human Anatomy, School of Basic Medicine Sciences, Beihua University, Jilin 132013, P.R.China

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通讯机构： [3]Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education Beijing 100053

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