Feedback modulation of endothelial cells promotes epithelial-mesenchymal transition and metastasis of osteosarcoma cells by Von Willebrand Factor release.
机构：[1]Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, China[2]Department of Hematology, Second Affiliated Hospital of Soochow University, Suzhou, China[3]Department of Orthopedics, Clinical Medical Research Center of Jiangsu Province, The First Affiliated Hospital of Soochow University, Suzhou, China[4]Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China[5]Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China
Endothelial cells (ECs), as a tumor niche cell, generate and secrete Von Willebrand factor (VWF) that is linked to osteosarcoma (OS) progression. However, the role and regulatory mechanisms of VWF that underpin OS progression remain unclear. Here, using a coculture system ex vivo, we showed that ECs promoted the epithelial-mesenchymal transition (EMT) process in OS cells via enhanced VWF secretion. VWF secreted by ECs directly contributed to OS EMT and metastasis by activating NF-κB signaling. In addition, OS cells exerted a feedback effect on ECs to promote VWF release via activation of phospholipase D 1 signaling, through which enhanced VWF secretion results in further tumor deterioration. To conclude, ECs served as a modulator and an effector of OS, accelerating OS exacerbation by VWF release.
? 2019 Wiley Periodicals, Inc.
基金：
Natural Science Foundation of Jiangsu
Province, Grant/Award Number:
BK20180053; National Natural Science
Foundation of China, Grant/Award
Numbers: 81600106, 81500107, 81600105;
Suzhou Science and Tecnology Project,
Grant/Award Number: SYS201610;
Jiangsu Provincial Medical Innovation
Team, Grant/Award Number:
CXTDA2017014
第一作者机构：[1]Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, China
共同第一作者：
通讯作者：
通讯机构：[3]Department of Orthopedics, Clinical Medical Research Center of Jiangsu Province, The First Affiliated Hospital of Soochow University, Suzhou, China
推荐引用方式(GB/T 7714)：
Jing Ling,Yu Sun,Jun Pan,et al.Feedback modulation of endothelial cells promotes epithelial-mesenchymal transition and metastasis of osteosarcoma cells by Von Willebrand Factor release.[J].Journal of cellular biochemistry.2019,doi:10.1002/jcb.28875.
APA：
Jing Ling,Yu Sun,Jun Pan,Huan Wang,Zhenni Ma...&Ling Liu.(2019).Feedback modulation of endothelial cells promotes epithelial-mesenchymal transition and metastasis of osteosarcoma cells by Von Willebrand Factor release..Journal of cellular biochemistry,,
MLA：
Jing Ling,et al."Feedback modulation of endothelial cells promotes epithelial-mesenchymal transition and metastasis of osteosarcoma cells by Von Willebrand Factor release.".Journal of cellular biochemistry .(2019)
