机构：[1]Departments of General Surgery,The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, PR China[2]Departments of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, PR China[3]Department of Clinical Laboratory, The Fifth People’s Hospital of Suzhou, Suzhou 215007, Jiangsu, PR China[4]Departments of Gastroenterology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, PR China[5]Department of Clinical Laboratory, The First Affiliated Hospital of Zhejiang University, Hangzhou 310006, Zhejiang, PR China.
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摘要：
The contribution of long non-coding RNAs (lncRNAs) to gastric cancer associated with Helicobacter pylori (H. pylori) infection remains largely unknown. Therefore, the present study aimed to investigate the expression of a lncRNA NR_026827 in gastric epithelial cells infected with H. pylori, and demonstrate its expression characteristic in gastric cancer.
Gastric epithelial cell line cells, GES-1, were cultured and infected with H. pylori. A microarray was used to analyze the lncRNA profile of gastric epithelial cells. Eighty fresh gastric cancer tissues and the paired adjacent non-cancerous tissue samples were randomly selected from patients. The expression of the lncRNA NR_026827 was investigated using quantitative real-time polymerase chain reaction (qRT-PCR).
The expression of several lncRNAs was significantly altered in GES-1 cells following infection with H. pylori. Of these lncRNAs, NR_026827 was dramatically down-regulated in GES-1 cells infected with H. pylori. In addition, the expression of NR_026827 was decreased in gastric cancer tissues in comparison to the corresponding adjacent non-cancerous tissues. Moreover, the expression of NR_026827 did not change significantly in different gastric cancer stages.
The lncRNA, NR_026827, is down-regulated in all stages of gastric cancer associated with H. pylori infection and could represent a potential biomarker for the diagnosis of gastric cancer.
基金：
This study was supported by the Science and Technology Program of Suzhou (SYS201618, SYS201551, SS201638, SZS201715), Jiangsu youth medical talents program (QN-866, 867), Six talent peaks project in Jiangsu Province (2016-WSN-112) and Gusu key health talent of Suzhou to Hong Du and Haifang Zhang.
第一作者机构：[1]Departments of General Surgery,The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, PR China
共同第一作者：
通讯作者：
通讯机构：[2]Departments of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, PR China
推荐引用方式(GB/T 7714)：
Fengyun Zhong,Meili Zhu,Kaijian Gao,et al.Low expression of the long non-coding RNA NR_026827 in gastric cancer.[J].American journal of translational research.2018,10(8):2706-2711.
APA：
Fengyun Zhong,Meili Zhu,Kaijian Gao,Ping Xu,Huan Yang...&Hong Du.(2018).Low expression of the long non-coding RNA NR_026827 in gastric cancer..American journal of translational research,10,(8)
MLA：
Fengyun Zhong,et al."Low expression of the long non-coding RNA NR_026827 in gastric cancer.".American journal of translational research 10..8(2018):2706-2711
