机构：[1]Department of Neurosurgery, Second Affiliated Hospital of Soochow University, Su-zhou, China[2]Department of Neurosurgery, Second Affiliated Hospital of Nantong University, Nan-tong, China
The role of AFAP1-AS1 in glioma is not fully known. This study aims at investigating the expression of AFAP1-AS1 in glioma and its underlying mechanism.
AFAP1-AS1 expressions in brain tissues from 52 cases of glioma and 5 cases of traumatic brain injury were assessed using quantitative fluorescence polymerase chain reaction (PCR). The clinicopathological features of glioma were first recorded. The correlation between AFAP1-AS1 expression and prognosis of glioma was discussed. AFAP1-AS1 expressions in the glioma cell lines were further detected. After knockdown of AFAP1-AS1 in U87MG and U251 glioma cells, cell invasion was assessed by transwell assay. MMP2 and MMP9 expressions in glioma cells, which were important indicators of cell invasive-ness, were determined by Western blot.
AFAP1-AS1 was upregulated in the glioma tissues compared with that in the control tissues, and the expression level was correlated with glioma grading and KPS scores. After knockdown of AFAP1-AS1, the invasion capacities of the glioma cells declined significantly, and the expressions of invasion-related proteins MMP2 and MMP9 also decreased significantly.
lncRNA AFAP1-AS1 can considerably facilitate the invasion of glioma cells and acts as an independent predictor of malignancy and prognosis, which may also serve as a potential therapeutic target.
基金：
The study was sponsored by a grant from the Scientific
Research Programme of Nantong (No.: GJZ16001).
第一作者机构：[1]Department of Neurosurgery, Second Affiliated Hospital of Soochow University, Su-zhou, China[2]Department of Neurosurgery, Second Affiliated Hospital of Nantong University, Nan-tong, China
通讯作者：
通讯机构：[1]Department of Neurosurgery, Second Affiliated Hospital of Soochow University, Su-zhou, China
推荐引用方式(GB/T 7714)：
Y. WANG,Q. LAN.Long non-coding RNA AFAP1-AS1 accelerates invasion and predicts poor prognosis of glioma.[J].European review for medical and pharmacological sciences.2018,22(16):5223-5229.doi:10.26355/eurrev_201808_15720.
APA：
Y. WANG&Q. LAN.(2018).Long non-coding RNA AFAP1-AS1 accelerates invasion and predicts poor prognosis of glioma..European review for medical and pharmacological sciences,22,(16)
MLA：
Y. WANG,et al."Long non-coding RNA AFAP1-AS1 accelerates invasion and predicts poor prognosis of glioma.".European review for medical and pharmacological sciences 22..16(2018):5223-5229
