Resveratrol Improves Endothelial Progenitor Cell Function through miR-138 by Targeting Focal Adhesion Kinase (FAK) and Promotes Thrombus Resolution In Vivo.
机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China
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摘要：
BACKGROUND Endothelial progenitor cells (EPCs) were found to be a potential therapeutic choice for low extremity deep vein thrombosis. The aim of our research was to investigate the effect of resveratrol (RSV) on EPCs that may promote thrombus resolution and its potential pathway. MATERIAL AND METHODS EPCs were pretreated with RSV and migration; angiogenesis were evaluated ex vivo. Expression of miR-138 and focal adhesion kinase (FAK) was also tested. A murine model of venous thrombosis was developed as an in vivo model. The effects of RSV treatment on mice with inferior venous thrombosis were evaluated. RESULTS We found that RSV increased EPCs migration and tube formation ex vivo. RSV significantly inhibited miR-138 expression. Moreover, we demonstrated that FAK was a target of miR-138 and revealed that FAK knockdown downregulated migration and angiogenesis of RSV-treated EPCs. In addition, RSV-induced EPCs promoted thrombus resolution in a murine model of venous thrombosis. CONCLUSIONS We found the first evidence that intravenous injection of RSV-treated EPCs enhanced thrombus resolution in vivo. RSV exerted its role by reducing miR-138 expression and therefore upregulated FAK.
基金：
This work was supported by grants from the National Natural Science Foundation of China (No.81400345, No.30972941),
Jiangsu Provincial Health Department’s Medical Science Program (H201211), Suzhou Science and Technology Development Plan
(SYSD-2015152), Graduate Research and Innovation Program in Colleges and Universities of Jiangsu Province (NO.KYCX17_1997)
第一作者机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China
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通讯作者：
通讯机构：[1]Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China
推荐引用方式(GB/T 7714)：
Yeqing Zhang,Xiaolong Du,Wendong Li,et al.Resveratrol Improves Endothelial Progenitor Cell Function through miR-138 by Targeting Focal Adhesion Kinase (FAK) and Promotes Thrombus Resolution In Vivo.[J].MEDICAL SCIENCE MONITOR.2018,24:951-960.
APA：
Yeqing Zhang,Xiaolong Du,Wendong Li,Hongfei Sang,Aimin Qian...&Chenglong Li.(2018).Resveratrol Improves Endothelial Progenitor Cell Function through miR-138 by Targeting Focal Adhesion Kinase (FAK) and Promotes Thrombus Resolution In Vivo..MEDICAL SCIENCE MONITOR,24,
MLA：
Yeqing Zhang,et al."Resveratrol Improves Endothelial Progenitor Cell Function through miR-138 by Targeting Focal Adhesion Kinase (FAK) and Promotes Thrombus Resolution In Vivo.".MEDICAL SCIENCE MONITOR 24.(2018):951-960
