机构：[1]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China[2]Department of Pathology, The First People’s Hospital of Kunshan, Jiangsu University, Suzhou 215300, Jiangsu, China[3]Department of Surgery, McMaster University, Hamilton, ON, Canada[4]Laboratory Center, The First People’s Hospital of Kunshan, Jiangsu University, Kunshan 215300, Jiangsu, China[5]Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China
出处：
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摘要：
Giant cell tumor of bone (GCTB) is a relatively rare tumor of bone, characterized by numerous multinucleated cells, severe osteolysis, and local recurrence.
To explore the role of S-phase kinase-interacting protein 2 (Skp2), cyclin-dependent kinase inhibitor p27, and the transcription factor E2F-1 expression in the development of GCTB, and the relationship of expression of these proteins with tumor recurrence.
Forty-four patients with GCTB were selected and demographic and clinical data were collected. The levels of Skp2, p27, and E2F-1 protein expression were immunohistochemically assessed in surgical specimens.
Skp2, p27, and E2F-1 proteins were detected in the nuclei of mononuclear stromal cells. Positive Skp2 expression was observed in 66% (29/44) of GCTB patient samples, and positive p27 expression was found in 39% (17/44) of samples. Within almost all GCTB patients, there was an inverse correlation between Skp2- and p27-positive tumor cells. Positive expression of E2F-1 was present in 28 of 44 (64%) patients. In addition, expression of skp2 and p27, infiltration of soft tissues, and surgical operation were significantly associated with recurrence in patients with GCTB.
The immunohistochemical assessment of Skp2, p27 and E2F-1 may be useful in the diagnosis of GCTB and prediction of its prognosis.
基金：
This study is supported by Social Developmental
Fund of Kunshan City, Jiangsu, China (KS1004).
第一作者机构：[1]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China[*1]Department of Orthopedics, The First People’s Hospital of Kunshan, Jiangsu University, No. 91 Qianjin Road (W), Kunshan 215300, Jiangsu, China.
通讯作者：
通讯机构：[*1]Department of Orthopedics, The First People’s Hospital of Kunshan, Jiangsu University, No. 91 Qianjin Road (W), Kunshan 215300, Jiangsu, China.
推荐引用方式(GB/T 7714)：
Chong Li,Xiaojuan Zheng,Michelle Ghert,et al.Expressions and clinical significance of factors related to giant cell tumor of bone.[J].International journal of clinical and experimental medicine.2015,8(12):22509-14.
APA：
Chong Li,Xiaojuan Zheng,Michelle Ghert,Hai Li,Bin Wang&Yizhong Feng.(2015).Expressions and clinical significance of factors related to giant cell tumor of bone..International journal of clinical and experimental medicine,8,(12)
MLA：
Chong Li,et al."Expressions and clinical significance of factors related to giant cell tumor of bone.".International journal of clinical and experimental medicine 8..12(2015):22509-14
