机构：[1]Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[2]Department of Orthopaedics, Liuzhou People’s Hospital, Liuzhou 545006, China[3]Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[4]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
出处：
ISSN：
摘要：
The aim of this experimental study was to evaluate the effect of intra-articular injection of Deoxycholic acid (DCA) on articular cartilage and subchondral bone following induction of knee Osteoarthritis (OA) in a rat model.
Twenty-four Sprague Dawley rats were randomized divided into 4 groups (n = 6). Eighteen of the 24 rats underwent surgical destabilization of the medial meniscus on the right knee joints to induce OA, were divided into 3 groups: DCA 30 mg/kg group, DCA 120 mg/kg group and OA group. The rats in DCA-treated groups were given intra-articular injections of DCA (30 mg/kg or 120 mg/kg) in the operated knees once per 3 days for 42 days. The rats in OA group given intra-articular injections of vehicle alone in the operated knees under the same conditions. The remaining 6 rats (sham-operation group) received sham operations on the right knee joints. 45 days postoperatively, all of the animals were euthanized for macroscopic, histological and radiographic analysis to evaluate the effect of DCA on OA and to determine its potential mechanisms.
The results showed that DCA attenuated the severity of OA by reducing macroscopic observation sores for femoral condyles and histological sores for articular cartilage. DCA also significantly decreased bone destruction and erosion of joint evaluated by radiographic examination. Furthermore, DCA could markedly reduce the release of MMP-1, MMP-3 and IL-1β in serum.
Intra-articular injection of DCA is beneficial for knee OA. It might repair and protect OA cartilage by delaying cartilage degeneration and impairing the function of inflammatory mediators. These findings highlight DCA might be a useful therapeutic agent for OA.
基金：
This work was supported by the grants from the Medical Research Projects of Health Department of Jiangsu Province (No. Z201304), National Natural Science Foundation of China (No. 81202348 and No. 81202394) and Jiangsu Province National Natural Science Funds (No. BK2012171), and Medical Research Projects of Health Department of Guangxi Zhuang Autonomous Region (No. Z2011291).
第一作者机构：[1]Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China[*1]Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, China.
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, China.[*2]Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China.
推荐引用方式(GB/T 7714)：
Zhaowei Yan,Jianbin Xiong,Chunyang Zhao,et al.Decreasing cartilage damage in a rat model of osteoarthritis by intra-articular injection of deoxycholic acid.[J].International journal of clinical and experimental medicine.2015,8(6):9038-45.
APA：
Zhaowei Yan,Jianbin Xiong,Chunyang Zhao,Chenhao Qin&Chunyan He.(2015).Decreasing cartilage damage in a rat model of osteoarthritis by intra-articular injection of deoxycholic acid..International journal of clinical and experimental medicine,8,(6)
MLA：
Zhaowei Yan,et al."Decreasing cartilage damage in a rat model of osteoarthritis by intra-articular injection of deoxycholic acid.".International journal of clinical and experimental medicine 8..6(2015):9038-45
