机构：[1]Department of Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China[2]Department of Gastroenterology, The Affiliated Jiangyin Hospital of Southeast University, Jiangyin 214400, China[3]School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China[4]Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.
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摘要：
Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer deaths worldwide. CD9 has been reported to play a critical role in cell motility, growth and metastasis of multiple cancers. The present study investigated the clinicopathological features of CD9, and its biological characteristics in ESCC.
Fifteen normal esophageal tissue specimens, fifty-three ESCC adjacent tissues and one hundred and four ESCC tissues were included in this study. Using immunohistochemistry (IHC), the expression levels of CD9 were evaluated among different samples. And its clinicopathological parameters and its prognostic factors were analyzed. Western blotting was used to measure CD9 expression and colony formation was performed to determine the effect of CD9 on cell growth in ESCC TE-1 cells.
Compared with normal esophageal tissues and tumor adjacent tissues, CD9 expression level is significantly higher in ESCC tissues. CD9 expression correlated with tumor stage (P=0.022) and lymph node metastasis (P=0.019) in ESCC patients. Furthermore, the small interfering RNA-mediated silencing of CD9 expression in TE-1 cells resulted in increased proliferation as evidenced by increased colony number and colony size.
CD9 expression is upregulated in ESCC tissues and its expression is correlated with tumor stage and lymph node metastasis in ESCC patients. CD9 suppresses the proliferation of TE-1 cells. CD9 may present a potential in tumor progression in ESCC.
基金：
This work is supported by the the National Natural Science Foundation of China (8137-2411), Jiangsu Provincial Special Program of Clinical Medical Science (BL2014040) and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
第一作者机构：[1]Department of Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, 1055 San Xiang Rd, Suzhou 215004, China.[*2]Medical College of Soochow University, No. 199 Ren’ai Rd, Suzhou 215123, China.
推荐引用方式(GB/T 7714)：
Jian Huan,Yi Gao,Jing Xu,et al.Overexpression of CD9 correlates with tumor stage and lymph node metastasis in esophageal squamous cell carcinoma.[J].International journal of clinical and experimental pathology.2015,8(3):3054-61.
APA：
Jian Huan,Yi Gao,Jing Xu,Wenjiong Sheng,Wei Zhu...&Ye Tian.(2015).Overexpression of CD9 correlates with tumor stage and lymph node metastasis in esophageal squamous cell carcinoma..International journal of clinical and experimental pathology,8,(3)
MLA：
Jian Huan,et al."Overexpression of CD9 correlates with tumor stage and lymph node metastasis in esophageal squamous cell carcinoma.".International journal of clinical and experimental pathology 8..3(2015):3054-61
