机构：[1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, No. 1055, Suzhou 215004, China[2]Department of General Surgery, The First People’s Hospital of Taicang City, Taicang Affiliated Hospital of Soochow University, No. 58, Taicang, Suzhou 215400, China[3]School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China[4]Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou 215123, China.
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摘要：
The prognosis of radioresistant colorectal cancer (CRC) is generally poor. Abnormal expression of microRNAs (miRNAs) is involved in the radiosensitivity of various tumor cells as these RNAs regulate biological signaling pathways. However, radioresistance-associated miRNAs in CRC have not yet been identified. In this study, we filtered out HCT116 and CCL-244 from seven CRC cell lines that showed the highest difference in radiosensitivity in a clonogenic assay. MiRNA sequencing identified 33 differentially expressed miRNAs (13 up-regulated and 20 down-regulated) in CCL-244 and 37 in HCT116 (20 up-regulated and 17 down-regulated) cells. MiR-100 was significantly down-regulated in CCL-244 cells after X-ray irradiation but not in HCT116 cells. Quantitative real-time PCR showed that the expression of miR-100 in CRC tissues was significantly lower than that in normal tissues. Thus, miR-100 seems to be involved in the radioresistance of CCL-244 cells. MiR-100 up-regulation sensitized CCL-244 cells to X-ray irradiation, which probably led to apoptosis and DNA double-strand breaks in these. In conclusion, to our knowledge, this is the first study to show that miR-100 may play an important role in regulating the radiosensitivity of CRC, and it may act as a new clinical target for CRC radiotherapy.
基金：
This work was partially supported by the National Natural Science Foundation of China (Grant No: 81172348, 81301933 and 81472917), Health Research Projects in Jiangsu Province (H201313), the projects of Suzhou Technology Bureau (SYSD2013090, SS0834) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
第一作者机构：[1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, No. 1055, Suzhou 215004, China
共同第一作者：
通讯作者：
通讯机构：[3]School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China[4]Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou 215123, China.[*1]Department of General Surgery, The Second Affiliated Hospital of Soochow University, No. 1055, Sanxian Rd, Suzhou 215004, China.
推荐引用方式(GB/T 7714)：
Xiao-Dong Yang,Xiao-Hui Xu,Shu-Yu Zhang,et al.Role of miR-100 in the radioresistance of colorectal cancer cells.[J].American journal of cancer research.2015,5(2):545-59.
APA：
Xiao-Dong Yang,Xiao-Hui Xu,Shu-Yu Zhang,Yong Wu,Chun-Gen Xing...&Jian-Ping Cao.(2015).Role of miR-100 in the radioresistance of colorectal cancer cells..American journal of cancer research,5,(2)
MLA：
Xiao-Dong Yang,et al."Role of miR-100 in the radioresistance of colorectal cancer cells.".American journal of cancer research 5..2(2015):545-59
