个人科研网厅 | | 帮助
首都医科大学宣武医院知识库
高级检索
当前位置： 首页 > 详情页

Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry.

| 认领 | 导出 | |

文献详情

资源类型：

收录情况： ◇ SCIE ◇ EI

作者：
Tingting Zhu [a] * ; Longjiang Xu [b] * ; Xiukun Xu [a] ; Zheng Wang[a] ; Jian Zhu [a] ; Qing Xie [a] ; Biyan Zhang [c] ; Yanping Wang [a] ; Linling Ju [a] ; Yun He [a] ; Xinshan Ye [e] ; Dapeng Zhou [d] ; Yunsen Li [a] ;
机构： [a]Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Jiangsu Laboratory of Infection Immunity, Soochow University, 199 Ren-Ai Road, Suzhou 215123, China [b]The Second Affiliated Hospital of Soochow University, Suzhou 215004, China [c]Department of Pharmaceutics, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China [d]Department of Melanoma Medical Oncology, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA [e]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100871, China
出处：
DOI： 10.1016/j.carres.2014.10.006
ISSN：

摘要：
Abnormal glycosphingolipids (GSLs) are expressed in many human tumors. These tumor-associated GSLs may have important roles in tumor progression. However, they are hard to be detected because of their low concentration and the limited availability of antibodies and lectins that recognize them. Thus, mass spectrometry is an effective method to analyze GSLs with high sensitivity. Here, we use electrospray ionization-linear ion trap quadrupole mass spectrometry (ESI-LTQ-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) to determine the structure of a certain GSL in human breast cancer tissue. To obtain the breast cancer-associated GSLs, we applied relative abundance contrast of GSLs and signal-to-noise ratio (SNR) analyses. We also used α1,2 fucosidase and fucosyltransferases as tools in the structural analysis. Based on this analysis, we identified the ion with m/z 1184 molecular ion as fucosyl-lactoceramide (Fuc-LacCer) with a C16 fatty acid ceramide. Quantitative analysis of GSLs revealed both Fuc-LacCer and Globo-H increased in breast cancer tissues. However, these two breast cancer-associated GSLs had different roles. The results of SNR analysis suggested the abnormal Fuc-LacCer is specific to breast cancer. The GSL profiling of breast cancer cells showed fucosyltransferase 1 contributed to the biosynthesis of Globo-H and Fuc-LacCer. In conclusion, MS analysis identified an accumulation of Fuc-LacCer in breast cancer tissue. Our findings provide GSL profiles of human breast cancer and develop an MS method for the study of cancer-associated GSLs. Copyright © 2014 Elsevier Ltd. All rights reserved.

基金：
Our work was supported by the National Natural Science Foundation of China (No. 31000370), the Natural Science Foundation of Jiangsu Province (No. BK2011288); the National Science and Technology key projects during the Twelfth Five-Year Plan Period of China (Nos. 2012ZX09401-014, 2012ZX09502001-001 and 2012CB822100), the Program for Changjiang Scholars and Innovative Research Team in University (Nos. IRT1075) and Jiangsu Provincial Innovative Research Team.
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类 | 4 区 化学
小类 | 3 区 应用化学 4 区 生化与分子生物学 4 区 有机化学
最新[2023]版：
大类 | 3 区 化学
小类 | 3 区 应用化学 3 区 有机化学 4 区 生化与分子生物学
JCR分区：
出版当年[2013]版：
Q2 CHEMISTRY, APPLIED Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Q3 CHEMISTRY, ORGANIC
最新[2023]版：
Q2 CHEMISTRY, APPLIED Q2 CHEMISTRY, ORGANIC Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2013版] 出版当年五年平均 出版前一年[2012版] 出版后一年[2014版]

第一作者：
第一作者机构： [a]Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Jiangsu Laboratory of Infection Immunity, Soochow University, 199 Ren-Ai Road, Suzhou 215123, China
共同第一作者：
通讯作者：
通讯机构： [a]Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Jiangsu Laboratory of Infection Immunity, Soochow University, 199 Ren-Ai Road, Suzhou 215123, China
推荐引用方式(GB/T 7714)：
Tingting Zhu,Longjiang Xu,Xiukun Xu,et al.Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry.[J].Carbohydrate research.2015,402:189-99.doi:10.1016/j.carres.2014.10.006.
APA：
Tingting Zhu,Longjiang Xu,Xiukun Xu,Zheng Wang,Jian Zhu...&Yunsen Li.(2015).Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry..Carbohydrate research,402,
MLA：
Tingting Zhu,et al."Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry.".Carbohydrate research 402.(2015):189-99

资源点击量：16461 今日访问量：0 总访问量：871 更新日期：2025-01-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 首都医科大学宣武医院 技术支持：重庆聚合科技有限公司 地址：北京市西城区长椿街45号宣武医院