机构：[1]Department of Orthopedics,The Second Affiliated Hospital of SooChow University,Suzhou, China[2]Department of Laboratory, The Second Affiliated Hospital ofSooChow University,[3]The Medical College of Soochow University,Suzhou,[4]Department of Orthopedics, The Peoples Hospital of Fuyang,Fuyang,[5]The Hong Kong Polytecbnic University, HongKong, China.
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摘要：
To address whether hepcidin functions in bone metabolism.
This study was carried out in the Laboratory of Radiation Medicine and Public Health of Soochow University, and the Laboratory of the Second Affiliated Hospital of Soochow University, Suzhou, China, from September 2009 to July 2010. The positive expression of ferroportin-1 (Fpn-1) was detected by reverse transcriptase-polymerase chain reaction. After the treatment with distilled water (control group) and hepcidin (25noml/L, 50noml/L, 100noml/L), the fluorescence intensity related to intracellular iron concentration of a human fetal osteoblast cell line (hFOB 1.19) was measured by a confocal laser scanning microscope. A 3-(4,5- dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide assay, and Von Kossa staining was performed to evaluate cell proliferation and mineralization in cultured hFOB 1.19 cells.
This study revealed a high level expression of Fpn-1 in hFOB 1.19. On the basis of which, it was found that 25noml/L, 50noml/L, 100noml/L hepcidin could promote the fluorescence intensity related to intracellular iron concentration and mineralization in hFOB 1.19 in a dose-dependent manner (p<0.05), but hepcidin had no effect on FOB 1.19 proliferation (p>0.05).
The hepcidin-ferroportin signal pathway may function in the osteoblast cell line of hFOB 1.19 cells. It is also suggested that cross-talk between iron and calcium homeostasis may play a role in bone metabolism in responding to hepcidin activation.
第一作者机构：[1]Department of Orthopedics,The Second Affiliated Hospital of SooChow University,Suzhou, China
通讯作者：
通讯机构：[1]Department of Orthopedics,The Second Affiliated Hospital of SooChow University,Suzhou, China
推荐引用方式(GB/T 7714)：
Peng Zhang,Youjia Xu,DongYang Zhao,et al.Increased intracellular iron and mineralization of cultured hFOB 1.19 cells following hepcidin activation through ferroportin-1.[J].Saudi medical journal.2010,31(12):1303-8.
APA：
Peng Zhang,Youjia Xu,DongYang Zhao,Yong Ma,Li Xiao...&Kai Li.(2010).Increased intracellular iron and mineralization of cultured hFOB 1.19 cells following hepcidin activation through ferroportin-1..Saudi medical journal,31,(12)
MLA：
Peng Zhang,et al."Increased intracellular iron and mineralization of cultured hFOB 1.19 cells following hepcidin activation through ferroportin-1.".Saudi medical journal 31..12(2010):1303-8
