24 case patients with acute necrotic pancreatis underwent dynamic CT examination at our hospital from 1990, of those patients suspected of pancreatic infection were performed direct bacteriologic sampling by CT-guided percutaneous aspiration. According to CT and bacterial results, we decided to whether operate or not. Meanwhile, multi-organ monitored and support therapy underwent. ANP mortalities reduced from 20.5% to 12.5%. We suggest that the dynamic CT and CT-guided aspiration is a safe, and rapid technique for distinguishing pancreatic infection from severe sterile pancreatitis, and may prove helpful in reducing the high morbidity and mortality associated with pancreatic infection.