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MicroRNA expression in the hippocampal CA1 region under deep hypothermic circulatory arrest.

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收录情况： ◇ SCIE ◇ 统计源期刊 ◇ CSCD-C

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机构： [1]Department of Anesthesiology, Xuanwu Hospital, Capital Medical University, Beijing, China [2]Institute of Geriatrics, Beijing, China [3]National Clinical Research Center for Geriatric Disorders, Beijing, China [4]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China [5]Cerebrovascular Research Center, Xuanwu Hospital, Capital Medical University, Beijing, China

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关键词： nerve regeneration cerebral protection deep hypothermic circulatory arrest gene ontology enrichment analysis microRNA hippocampus post-transcriptional expression microarray bioinformatics neural regeneration

摘要：

Using deep hypothermic circulatory arrest, thoracic aorta diseases and complex heart diseases can be subjected to corrective procedures. However, mechanisms underlying brain protection during deep hypothermic circulatory arrest are unclear. After piglet models underwent 60 minutes of deep hypothermic circulatory arrest at 14°C, expression of microRNAs (miRNAs) was analyzed in the hippocampus by microarray. Subsequently, TargetScan 6.2, RNA22 v2.0, miRWalk 2.0, and miRanda were used to predict potential targets, and gene ontology enrichment analysis was carried out to identify functional pathways involved. Quantitative reverse transcription-polymerase chain reaction was conducted to verify miRNA changes. Deep hypothermic circulatory arrest altered the expression of 35 miRNAs. Twenty-two miRNAs were significantly downregulated and thirteen miRNAs were significantly upregulated in the hippocampus after deep hypothermic circulatory arrest. Six out of eight targets among the differentially expressed miRNAs were enriched for neuronal projection (cyclin dependent kinase, CDK16 and SLC1A2), central nervous system development (FOXO3, TYRO3, and SLC1A2), ion transmembrane transporter activity (ATP2B2 and SLC1A2), and interleukin-6 receptor binding (IL6R) - these are the key functional pathways involved in cerebral protection during deep hypothermic circulatory arrest. Quantitative reverse transcription-polymerase chain reaction confirmed the results of microarray analysis. Our experimental results illustrate a new role for transcriptional regulation in deep hypothermic circulatory arrest, and provide significant insight for the development of miRNAs to treat brain injuries. All procedures were approved by the Animal Care Committee of Xuanwu Hospital, Capital Medical University, China on March 1, 2017 (approval No. XW-INI-AD2017-0112).

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出版当年[2018]版：

大类 | 4 区医学

小类 | 4 区细胞生物学 4 区神经科学

最新[2023]版：

大类 | 2 区医学

小类 | 3 区细胞生物学 3 区神经科学

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出版当年[2017]版：

Q3 NEUROSCIENCES Q3 CELL BIOLOGY

最新[2023]版：

Q1 NEUROSCIENCES Q2 CELL BIOLOGY

影响因子： 5.9 最新[2023版] 5.2 最新五年平均 2.234 出版当年[2017版] 1.599 出版当年五年平均 1.769 出版前一年[2016版] 2.472 出版后一年[2018版]

第一作者：

第一作者机构： [1]Department of Anesthesiology, Xuanwu Hospital, Capital Medical University, Beijing, China [2]Institute of Geriatrics, Beijing, China [3]National Clinical Research Center for Geriatric Disorders, Beijing, China

通讯作者：

通讯机构： [1]Department of Anesthesiology, Xuanwu Hospital, Capital Medical University, Beijing, China [2]Institute of Geriatrics, Beijing, China [3]National Clinical Research Center for Geriatric Disorders, Beijing, China [4]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China [5]Cerebrovascular Research Center, Xuanwu Hospital, Capital Medical University, Beijing, China

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MicroRNA expression in the hippocampal CA1 region under deep hypothermic circulatory arrest.

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