机构：[1]Department of Urology, Xuanwu Hospital of The Capital Medical University, Xuanwu Hospital, No.45,Changchun Street, Xicheng district, Beijing, P. R. China.泌尿外科首都医科大学宣武医院[2]General Practice, Beijing Xicheng District White Paper Community Health Service Center, No.4 Xinan Zhongli, Xicheng district, Beijing, P. R. China.[3]Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095, liberation avenue, Wuhan, 430030, Hubei, P. R. China
Bladder cancer (BC) is the ninth most frequent malignancy and the thirteenth leading cause of cancer-related death worldwide. CDKN1A-interacting zinc finger protein 1 (CIZ1) is involved in the development of various cancers, while its role in BC remains unclear. In this study, we found that CIZ1 was up-regulated in BC tissues and cells. Knockdown of CIZ1 suppressed the proliferation and growth of T24 and 5637 cells. Apoptosis and caspase 3/7 activity were enhanced after CIZ1 silencing in both cells. At the molecular level, PCNA, Ki67, HIF-1α, survivin, CCND1 and CCNB1 were down-regulated, and caspase-3, p53, p21, caspase-9 were up-regulated by CIZ1 knockdown. This suggested that silencing of CIZ1 suppressed BC cell proliferation through inducing apoptosis and reducing cell cycle progression. Our study suggests that targeting CIZ1 maybe a potential therapeutic strategy for BC.
Copyright ? 2019. Published by Elsevier B.V.
基金：
This project was supported by funds from the National Natural Science Foundation of China
(81270851 & 31570988).
第一作者机构：[1]Department of Urology, Xuanwu Hospital of The Capital Medical University, Xuanwu Hospital, No.45,Changchun Street, Xicheng district, Beijing, P. R. China.
通讯作者：
通讯机构：[*1]Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095, liberation avenue, Wuhan, 430030, Hubei, P. R. China
推荐引用方式(GB/T 7714)：
Xiaosong Chen,Ping Wang,Shenghan Wang,et al.CIZ1 knockdown suppresses the proliferation of bladder cancer cells by inducing apoptosis.[J].Gene.2019,719:143946.doi:10.1016/j.gene.2019.143946.
APA：
Xiaosong Chen,Ping Wang,Shenghan Wang,Jin Li,Tongwen Ou&Xiaoyong Zeng.(2019).CIZ1 knockdown suppresses the proliferation of bladder cancer cells by inducing apoptosis..Gene,719,
MLA：
Xiaosong Chen,et al."CIZ1 knockdown suppresses the proliferation of bladder cancer cells by inducing apoptosis.".Gene 719.(2019):143946
