Preparation of Small-Diameter Tissue-Engineered Vascular Grafts Electrospun from Heparin End-Capped PCL and Evaluation in a Rabbit Carotid Artery Replacement Model.
机构：[1]School of Materials Science and Engineering Beijing Institute of Technology Beijing 100081, China[2]Beijing Key Laboratory of Pre-clinic Research and Evaluation for Cardiovascular Implant Materials Fuwai Hospital National Cardiovascular Center Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100037, China[3]Department of Vascular Surgery Xuanwu Hospital and Institute of Vascular Surgery Capital Medical University Beijing 100053, China血管外科首都医科大学宣武医院
Aiming to construct small diameter (ID <6 mm) off-the-shelf tissue-engineered vascular grafts, the end-group heparinizd poly(ε-caprolactone) (PCL) is synthesized by a three-step process and then electrospun into an inner layer of double-layer vascular scaffolds (DLVSs) showing a hierarchical double distribution of nano- and microfibers. Afterward, PCL without the end-group heparinization is electrospun into an outer layer. A steady release of grafted heparin and the existence of a glycocalyx structure give the grafts anticoagulation activity and the conjugation of heparin also improves hydrophilicity and accelerates degradation of the scaffolds. The DLVSs are evaluated in six rabbits via a carotid artery interpositional model for a period of three months. All the grafts are patent until explantation, and meanwhile smooth endothelialization and fine revascularization are observed in the grafts. The composition of the outer layer of scaffolds exhibits a significant effect on the aneurysm dilation after implantation. Only one aneurysm dilation is detected at two months and no calcification is formed in the follow-up term. How to prevent aneurysms remains a challenging topic.
? 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
基金：
National Key R&D Program
of China (2017YFC1104101).
第一作者机构：[1]School of Materials Science and Engineering Beijing Institute of Technology Beijing 100081, China
通讯作者：
推荐引用方式(GB/T 7714)：
Xin Jin,Xue Geng ,Liujun Jia ,et al.Preparation of Small-Diameter Tissue-Engineered Vascular Grafts Electrospun from Heparin End-Capped PCL and Evaluation in a Rabbit Carotid Artery Replacement Model.[J].Macromolecular bioscience.2019,19(8):e1900114.doi:10.1002/mabi.201900114.
APA：
Xin Jin,Xue Geng,,Liujun Jia,,Zeqin Xu,Lin Ye,...&Zeng-Guo Feng.(2019).Preparation of Small-Diameter Tissue-Engineered Vascular Grafts Electrospun from Heparin End-Capped PCL and Evaluation in a Rabbit Carotid Artery Replacement Model..Macromolecular bioscience,19,(8)
MLA：
Xin Jin,et al."Preparation of Small-Diameter Tissue-Engineered Vascular Grafts Electrospun from Heparin End-Capped PCL and Evaluation in a Rabbit Carotid Artery Replacement Model.".Macromolecular bioscience 19..8(2019):e1900114
