机构：[1]Institute of Cerebrovascular Diseases Research, Department of Neurology, and Department of Neurosurgery of Xuanwu Hospital, Capital Medical University, Beijing, China 神经内科神经外科首都医科大学宣武医院[2]Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China [3]Health Management Center, Xuanwu Hospital of Capital Medical University, China 首都医科大学宣武医院[4]Beijing Institute for Brain Disorders, Beijing, China
Moyamoya disease (MMD) is a rare cerebrovascular disorder with higher incidences in Eastern Asian countries but the natural course remains uncertain. Circular RNAs (circRNAs) have been implicated in brain disorders, but their role in the development of MMD is unclear. Neutrophil depletion has been shown to affect stem cell migration, fate, and therapeutic outcomes. We investigated the circRNAs expression profile of neutrophil transcriptome in patients with asymptomatic MMD. Microarray based circRNAs profiling was determined between neutrophil samples from patients with asymptomatic MMD and healthy subjects. The microarray results were followingly confirmed by quantitative reverse-transcription PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses (KEGG) were adopted for annotation and predicting the functions of differentially expressed circRNAs. From this comparative circRNA microarray analysis of neutrophil samples from patients with asymptomatic MMD and healthy subjects, 123 circRNAs were identified differentially expressed between the two groups. Of these, 54 were upregulated and 69 were downregulated compared to controls (fold change >2.0 and P?<?0.05). GO and KEGG analyses revealed that the differentially expressed circRNAs were mainly involved in immune responses, angiogenesis and metabolism in asymptomatic MMD. Besides, the hypoxia inducing factor-1α signaling pathway was found to be the critical pathway involved in the angiogenesis of disease pathogenesis. This is a pilot study on the neutrophils from the asymptomatic MMD and aberrantly expressed circRNAs in the profiling obtained by high-throughput microarray may help provide insights into MMD.
Copyright ? 2019. Published by Elsevier B.V.
基金：
This project was supported by the Natural Science Foundation in
China (81771413, 81601157, 81571280).
第一作者机构：[1]Institute of Cerebrovascular Diseases Research, Department of Neurology, and Department of Neurosurgery of Xuanwu Hospital, Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Neurosurgery of Xuanwu Hospital, Capital Medical University, Beijing 100053, China[*2]Institute of Cerebrovascular Disease Research and Department of Neurology, Xuanwu Hospital of Capital Medical University, 45 Changchun Street, Beijing 100053, China
推荐引用方式(GB/T 7714)：
Ma Qingfeng,Li Lingzhi,Yu Bingxin,et al.Circular RNA profiling of neutrophil transcriptome provides insights into asymptomatic Moyamoya disease.[J].Brain research.2019,1719:104-112.doi:10.1016/j.brainres.2019.05.033.
APA：
Ma Qingfeng,Li Lingzhi,Yu Bingxin,Jiao Liqun,Han Ziping...&Luo Yumin.(2019).Circular RNA profiling of neutrophil transcriptome provides insights into asymptomatic Moyamoya disease..Brain research,1719,
MLA：
Ma Qingfeng,et al."Circular RNA profiling of neutrophil transcriptome provides insights into asymptomatic Moyamoya disease.".Brain research 1719.(2019):104-112
