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Hypoxia conditioning enhances neuroprotective effects of aged human bone marrow mesenchymal stem cell-derived conditioned medium against cerebral ischemia in vitro.

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机构： [a]Department of Neurobiology, Capital Medical University, Beijing 100069, China [b]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing 100053, China [c]Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China [d]Department of Hematology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China. [e]Department of Neurosurgery and Brain Repair, University of South Florida, Tampa, FL, USA [f]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

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Therapeutic transplantation of autologous bone marrow mesenchymal stem cells (BMSCs) holds great promise for ischemic stroke, yet the efficacy is negatively impacted by aging. Here, we examined whether hypoxia conditioning could enhance aged human BMSCs-induced neuroprotection via secretome action. Primary cultured mouse neurons were exposed to oxygen glucose deprivation (OGD) to mimic ischemic stroke in vitro, then randomized into a hypoxia conditioned aged human BMSCs-conditioned medium (BMSC-hypoCM) versus normoxia conditioned (BMSC-norCM). After 22 hours of reperfusion, cell viability was significantly increased in neurons treated with BMSC-hypoCM rather than BMSC-norCM. ELISA revealed that hypoxia conditioning enhanced vascular endothelial growth factor (VEGF) release into BMSC-derived CM. Blocking the VEGF receptor negated BMSC-hypoCM-induced protection for neurons against OGD insult. Altogether, our data indicates that hypoxia conditioning improves aged human BMSCs' therapeutic efficacy for neurons with ischemic challenge, in part via promoting secretion of VEGF. Copyright © 2019. Published by Elsevier B.V.

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出版当年[2018]版：

大类 | 3 区医学

小类 | 3 区神经科学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区神经科学

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出版当年[2017]版：

Q2 NEUROSCIENCES

最新[2023]版：

Q3 NEUROSCIENCES

影响因子： 2.7 最新[2023版] 2.9 最新五年平均 3.125 出版当年[2017版] 2.929 出版当年五年平均 2.746 出版前一年[2016版] 2.929 出版后一年[2018版]

第一作者：

第一作者机构： [a]Department of Neurobiology, Capital Medical University, Beijing 100069, China [b]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing 100053, China [c]Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China

通讯作者：

通讯机构： [b]Beijing Key Laboratory of Hypoxia Conditioning Translational Medicine, Beijing 100053, China [c]Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China [f]Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

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