机构：[1]School of Pharmacy, Minzu University of China, 27 South Street, Zhongguancun, Beijing 100081, China.[2]Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing 100081, China.[3]Center for Drug Evaluation, China Food and Drug Administration, Beijing 100081, China.[4]Department of Histology and Embryology, Xinxiang Medical University, Xinxiang 453003, Henan, China.[5]Department of Pathology, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China.医技科室职能科室病理科临床流行病与循证医学中心首都医科大学附属北京儿童医院
BackgroundRecent studies have found gut microbiota to be closely associated with onset and perpetuation of UC. Currently, studies about gut microbiota have mainly covered samples collected from the intestinal lumen. However, the luminal flora is only part of the gut microbiota. Studies of the changes in mucosal flora under pathological conditions have been lacking. In this study, we investigated the correlation between the onset of UC and flora changes in different intestinal layers.MethodsThe dextran sulfate sodium(DSS)-induced UC model was established by exposing mice to cycles of DSS. The luminal contents, an inner mucus layer, and outer mucus layer were harvested under sterile conditions. The samples were then analyzed using high-throughput sequencing of 16S rRNA V3+V4 amplicons. The colonic microbiota composition and diversity were analyzed and compared using MetaStat, LefSe, multivariate analysis of variance, and spatial statistics.ResultsThe DSS-induced UC mouse model was successfully established. The diversity of the microbiota from luminal content, the outer mucus layer, and inner mucus layer were significantly different in both control and UC model groups. The statistically different OTUs belonged to Lachnospiraceae and Ruminococcaceae families within the order Clostridiales were mainly localized to the outer mucus layer.ConclusionsThe alterations in flora composition and diversity mainly occurred in the colonic outer mucus layer. The change of flora in the colonic mucus layers is of great significance in the understanding of common features of gut flora in IBD and the understanding of the relationship between gut flora and disease progression.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81473796, 81774449]; Program for Changjiang Scholars and Innovative Research Team in University PCSIRTProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT 13R63]; Ministry of Education, ChinaMinistry of Education, China
第一作者机构：[1]School of Pharmacy, Minzu University of China, 27 South Street, Zhongguancun, Beijing 100081, China.[2]Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing 100081, China.
共同第一作者：
通讯作者：
通讯机构：[1]School of Pharmacy, Minzu University of China, 27 South Street, Zhongguancun, Beijing 100081, China.[2]Key Laboratory of Ethnomedicine (Minzu University of China), Ministry of Education, Beijing 100081, China.
推荐引用方式(GB/T 7714)：
Pei Ling-yan,Ke Yu-shi,Zhao Huan-hu,et al.Role of colonic microbiota in the pathogenesis of ulcerative colitis[J].BMC GASTROENTEROLOGY.2019,19(1):-.doi:10.1186/s12876-019-0930-3.
APA：
Pei, Ling-yan,Ke, Yu-shi,Zhao, Huan-hu,Wang, Lin,Jia, Chao...&Li, Shu-chun.(2019).Role of colonic microbiota in the pathogenesis of ulcerative colitis.BMC GASTROENTEROLOGY,19,(1)
MLA：
Pei, Ling-yan,et al."Role of colonic microbiota in the pathogenesis of ulcerative colitis".BMC GASTROENTEROLOGY 19..1(2019):-
