Feasibility and Performance of Loop-Mediated Isothermal Amplification Assay in the Diagnosis of Pulmonary Tuberculosis in Decentralized Settings in Eastern China
机构：[1]Qingdao Municipal Center for Disease Control and Prevention, Qingdao, China[2]Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, MOE Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China临床科室科研平台职能科室耳鼻咽喉头颈外科临床流行病与循证医学中心儿科研究所首都医科大学附属北京儿童医院
Early diagnosis is essential for the control and prevention of tuberculosis (TB). The objective of this study was to investigate the feasibility and performance of loop-mediated isothermal amplification (LAMP) in the diagnosis of pulmonary TB in county-level microscopy centers in Qingdao, Eastern China. A total of 523 presumptive TB patients were consecutively recruited between July 2017 and April 2018, and 22 patients were excluded from the analysis. Of 102 culture-positive cases, TB-LAMP identified 91 cases, demonstrating a sensitivity of 89.2%. In comparison, the sensitivity of routine smear microscopy was 69.6% (71/102), which was significantly lower than that of TB-LAMP (P=0.001). In addition, TB-LAMP sensitivities in smear-positive and smear-negative samples were 98.6% and 67.7%, respectively. In conclusion, our data demonstrate that TB-LAMP outperforms conventional smear microscopy in TB diagnosis, which could be used as an alternative method for smear microscopy in resource-limited settings in China.
基金：
Qingdao Municipal Health and Family Planning Commission
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2023]版：
无
JCR分区：
出版当年[2017]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Qingdao Municipal Center for Disease Control and Prevention, Qingdao, China
通讯作者：
通讯机构：[1]Qingdao Municipal Center for Disease Control and Prevention, Qingdao, China[2]Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, MOE Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China
推荐引用方式(GB/T 7714)：
Wang Zhongdong,Sun Haiyan,Ren Zhisheng,et al.Feasibility and Performance of Loop-Mediated Isothermal Amplification Assay in the Diagnosis of Pulmonary Tuberculosis in Decentralized Settings in Eastern China[J].BIOMED RESEARCH INTERNATIONAL.2019,2019:-.doi:10.1155/2019/6845756.
APA：
Wang, Zhongdong,Sun, Haiyan,Ren, Zhisheng,Xue, Bai,Lu, Jie&Zhang, Huaqiang.(2019).Feasibility and Performance of Loop-Mediated Isothermal Amplification Assay in the Diagnosis of Pulmonary Tuberculosis in Decentralized Settings in Eastern China.BIOMED RESEARCH INTERNATIONAL,2019,
MLA：
Wang, Zhongdong,et al."Feasibility and Performance of Loop-Mediated Isothermal Amplification Assay in the Diagnosis of Pulmonary Tuberculosis in Decentralized Settings in Eastern China".BIOMED RESEARCH INTERNATIONAL 2019.(2019):-
