Objective: To investigate whether there were differences in Th17 cells mediated immunological responses among asthmatics with or without allergic rhinitis. Methods: A case-control comparison was conducted in a cohort of 67 children with asthma (AS), 50 children with allergic rhinitis (AR), 52 children with both AS and AR (ASR), 25 infectious rhinitis (IR), and 55 healthy controls (H). The percentages of circulating Th17 cells were determined by flow cytometry. The Th2- and Th17-related cytokines in plasma and culture supernatants were measured by enzyme-linked immunosorbent assay. The effect of proinflammation cytokine IL-17E on Th2 cytokines production from human T helper (Th) lymphocytes was analyzed. Results: (1) A inter-group comparison revealed that Th17 cells levels were highest in ASR group [(0.89% +/- 0.27) %], followingby AS group [(0.82 +/- 0.29) %] and AR group[(0.78 +/- 0.17) %] (P < 0.05). (2) After in-vitro stimulation with house dust mite (HDM) antigen, the levels of IL-4 and IL-17E in culture supernatants of PBMCs from allergic children (AS group, AR group and ASR group) were significantly enhanced. (3) The release of Th2 cytokines from IL-17E treated Th cells of allergic children (AS group, AR group and ASR group) were significantly induced, no similar result was observed in IR group and HC group. Conclusion: Our findings preliminarily revealed that Th17 cell and its related cytokines might be involved in pathogenesis of airway inflammation diseases, and also presenting varying immunological characteristics among asthmatic children with or without allergic rhinitis.
基金：
Beijing Municipal Natural Science FoundationBeijing Natural Science Foundation [7172074]; Beijing Municipal Administration of Hospitals Incubating Program [PX2018049]
第一作者机构：[1]Allergy Department, Beijing Children’s hospital, Capital Medical University, Beijing, China, No.56 Nanlishi Road, Xicheng District, 100045, Beijing P.R.China
通讯作者：
通讯机构：[1]Allergy Department, Beijing Children’s hospital, Capital Medical University, Beijing, China, No.56 Nanlishi Road, Xicheng District, 100045, Beijing P.R.China[*1]Allergy Department, Beijing Children’s Hospital, Capital Medical University, No.56 Nanlishi Road, Xicheng District,100045, Beijing P.R.China
推荐引用方式(GB/T 7714)：
Miao Qing ,Liu Yongge ,Xu Wei ,et al.Comparison of Th17 cells mediated immunological response among asthmatic children with or without allergic rhinitis[J].ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY.2019,37(2):65-72.doi:10.12932/AP-310517-0097.
APA：
Miao Qing,,Liu Yongge,,Xu Wei,,Wang Yan,,Li Zhen,...&Xiang Li.(2019).Comparison of Th17 cells mediated immunological response among asthmatic children with or without allergic rhinitis.ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY,37,(2)
MLA：
Miao Qing,,et al."Comparison of Th17 cells mediated immunological response among asthmatic children with or without allergic rhinitis".ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY 37..2(2019):65-72
