机构:[1]Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Tian Tan Xi Li No.4, Beijing 100050, People’s Republic of China.[2]Department of Orthodontics, Capital Medical University School of Stomatology, Beijing, People’s Republic of China.[3]Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University, Beijing, People’s Republic of China首都医科大学附属天坛医院
Background: Critical tissues that undergo regeneration in periodontal tissue are of mesenchymal origin; thus, investigating the regulatory mechanisms underlying the fate of periodontal ligament stem cells could be beneficial for application in periodontal tissue regeneration. Nitric oxide (NO) regulates many biological processes in developing embryos and adult stem cells. The present study was designed to investigate the effects of NO on the function of human periodontal ligament stem cells (PDLSCs) as well as to elucidate the underlying molecular mechanisms. Methods: Immunofluorescent staining and flow cytometry were used for stem cell identification. Western blot, reverse transcription polymerase chain reaction (RT-PCR), immunofluorescent staining, and flow cytometry were used to examine the expression of NO-synthesizing enzymes. The proliferative capacity of PDLSCs was determined by EdU assays. The osteogenic potential of PDLSCs was tested using alkaline phosphatase (ALP) staining, Alizarin Red staining, and calcium concentration detection. Oil Red O staining was used to analyze the adipogenic ability. Western blot, RT-PCR, and staining were used to examine the signaling pathway. Results: Human PDLSCs expressed both inducible NO synthase (iNOS) and endothelial NO synthase (eNOS) and produced NO. Blocking the generation of NO with the NOS inhibitor L-N-G-monomethyl arginine (L-NMMA) had no influence on PDLSC proliferation and apoptosis but significantly attenuated the osteogenic differentiation capacity and stimulated the adipogenic differentiation capacity of PDLSCs. Increasing the physiological level of NO with NO donor sodium nitroprusside (SNP) significantly promoted the osteogenic differentiation capacity but reduced the adipogenic differentiation capacity of PDLSCs. NO balances the osteoblast and adipocyte lineage differentiation in periodontal ligament stem cells via the c-Jun N-terminal kinase (JNK)/mitogen-activated protein kinase (MAPK) signaling pathway. Conclusions: NO is essential for maintaining the balance between osteoblasts and adipocytes in PDLSCs via the JNK/MAPK signaling pathway.
基金:
National Nature Science Foundation of ChinaNational Natural Science Foundation of China [81470751, 81600891, 81600829]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7172087]; Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201703]; Beijing Baiqianwan Talents Project [2017A17]
语种:
外文
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2017]版:
大类|2 区医学
小类|2 区医学:研究与实验3 区细胞生物学
最新[2023]版:
大类|2 区医学
小类|2 区细胞与组织工程2 区细胞生物学2 区医学:研究与实验
JCR分区:
出版当年[2016]版:
Q1MEDICINE, RESEARCH & EXPERIMENTALQ2CELL BIOLOGY
最新[2023]版:
Q1CELL & TISSUE ENGINEERINGQ1CELL BIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构:[1]Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Tian Tan Xi Li No.4, Beijing 100050, People’s Republic of China.
共同第一作者:
通讯作者:
通讯机构:[1]Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Tian Tan Xi Li No.4, Beijing 100050, People’s Republic of China.
推荐引用方式(GB/T 7714):
Yang Shan,Guo Lijia,Su Yingying,et al.Nitric oxide balances osteoblast and adipocyte lineage differentiation via the JNK/MAPK signaling pathway in periodontal ligament stem cells[J].STEM CELL RESEARCH & THERAPY.2018,9(1):-.doi:10.1186/s13287-018-0869-2.
APA:
Yang, Shan,Guo, Lijia,Su, Yingying,Wen, Jing,Du, Juan...&Liu, Yi.(2018).Nitric oxide balances osteoblast and adipocyte lineage differentiation via the JNK/MAPK signaling pathway in periodontal ligament stem cells.STEM CELL RESEARCH & THERAPY,9,(1)
MLA:
Yang, Shan,et al."Nitric oxide balances osteoblast and adipocyte lineage differentiation via the JNK/MAPK signaling pathway in periodontal ligament stem cells".STEM CELL RESEARCH & THERAPY 9..1(2018):-