机构：[1]Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, 100050 Beijing, China.研究所北京市神经外科研究所首都医科大学附属天坛医院[2]Beijing Key Laboratory of Neuromodulation, Beijing Municipal Science and Technology Commission, 100050 Beijing, China.[3]Department of Neurosurgery, Beijing Children’s Hospital, Capital Medical University, 100045 Beijing, China.临床科室神经外科首都医科大学附属北京儿童医院[4]Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 100050 Beijing, China.重点科室诊疗科室神经外科首都医科大学附属天坛医院[5]Department of Neurology, University of Florida, Gainesville, Florida, 32607, USA.[6]Department of Pathology, School of Basic Medical Sciences, Capital Medical University, 100069 Beijing, China
Temporal lobe epilepsy (TLE) is one of the most common types of intractable epilepsy, characterized by hippocampal neuron damage and hippocampal sclerosis. Long noncoding RNAs (lncRNAs) have been increasingly recognized as posttranscriptional regulators. However, their expression levels and functions in TLE remain largely unknown. In the present study, TLE rat model is used to explore the expression profiles of lncRNAs in the hippocampus of epileptic rats using microarray analysis. Our results demonstrate that H19 is the most pronouncedly differentiated lncRNA, significantly upregulated in the latent period of TLE. Moreover, the in vivo studies using gain- and loss-of-function approaches reveal that the overexpression of H19 aggravates SE-induced neuron apoptosis in the hippocampus, while inhibition of H19 protects the rats from SE-induced cellular injury. Finally, we show that H19 might function as a competing endogenous RNA to sponge microRNA let-7b in the regulation of cellular apoptosis. Overall, our study reveals a novel lncRNA H19-mediated mechanism in seizure-induced neural damage and provides a new target in developing lncRNA based strategies to reduce seizure-induced brain injury.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81471315]
第一作者机构：[1]Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, 100050 Beijing, China.[2]Beijing Key Laboratory of Neuromodulation, Beijing Municipal Science and Technology Commission, 100050 Beijing, China.
通讯作者：
通讯机构：[1]Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, 100050 Beijing, China.[2]Beijing Key Laboratory of Neuromodulation, Beijing Municipal Science and Technology Commission, 100050 Beijing, China.[6]Department of Pathology, School of Basic Medical Sciences, Capital Medical University, 100069 Beijing, China
推荐引用方式(GB/T 7714)：
Chun-Lei Han,Ming Ge,Yun-Peng Liu,et al.Long non-coding RNA H19 contributes to apoptosis of hippocampal neurons by inhibiting let-7b in a rat model of temporal lobe epilepsy[J].CELL DEATH & DISEASE.2018,9(6):-.doi:10.1038/s41419-018-0496-y.
APA：
Chun-Lei Han,Ming Ge,Yun-Peng Liu,Xue-Min Zhao,Kai-Liang Wang...&Fan-Gang Meng.(2018).Long non-coding RNA H19 contributes to apoptosis of hippocampal neurons by inhibiting let-7b in a rat model of temporal lobe epilepsy.CELL DEATH & DISEASE,9,(6)
MLA：
Chun-Lei Han,et al."Long non-coding RNA H19 contributes to apoptosis of hippocampal neurons by inhibiting let-7b in a rat model of temporal lobe epilepsy".CELL DEATH & DISEASE 9..6(2018):-
生物学