机构：[1]State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Science, Beijing, China.[2]Anhui Medical University, Anhui, China.[3]The Affiliated Hospital Of Military Medical Science, Beijing, China.[4]Department of Infectious Disease, Beijing Children’s Hospital, Capital Medical University, Beijing, China.职能科室临床流行病与循证医学中心首都医科大学附属北京儿童医院[5]Chinese People’s Liberation Army General Hospital (301 Hospital), Beijing, China.
Bloodstream infections (BSIs) are often life-threatening, and rapid identification is critical. Here, we developed a TaqMan array card (TAC) assay to detect pathogens in BSI specimens. The TAC included 30 primer/probe pairs targeting 27 species and 3 controls. Reverse transcription and 0.1% blue dextran 2000 increased the TAC assay efficiency. The primer/probe pairs had a limit of detection of 100-102 CFU/mL and a specificity of 100%. For whole blood specimens, the TAC assay showed a sensitivity and specificity of 79.4% and 99.69%, respectively. For blood culture samples, the TAC assay showed a sensitivity and specificity of 100% and 99.67%, respectively. The TAC assay could be a promising method for early detection of bloodstream infection.
基金：
Chinese State Key Project Specialized for Infectious Disease [2018ZX10712001-015, 2018ZX10732401-001-008, 2018ZX10711001-003, AWS15J006]; State Key Laboratory of Pathogen and Biosecurity (Academy of Military Medical Science) [SKLPBS1421]; Chinese State Key Project Specialized for new drugs [2016ZX09J16105-002-002]; Academy of Military Medical Sciences Innovation Fund [2017CXJJ06]
