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PGK1 as an immune target in Kawasaki disease

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机构: [1]112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, and [2]Department of Pediatrics, Beijing Children’s Hospital Affiliated to Capital Medical University, Beijing, China
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关键词: antigenicity analysis immune target Kawasaki disease PGK1

摘要:
Kawasaki disease (KD) is an immune-mediated vasculitis with symptoms that mimic febrile illness; the immune origin has been suggested but never proved. First, cell chip technology was used to screen immune targets cells. With the indirect immunofluorescence assay we found that the HeLa cell chip could be recognized by KD patient serum samples. The target cell proteome was extracted as antigens, and antigen recognition reaction was performed using the patients' serum as antibodies and the detected target protein was detected and identified as phosphoglycerate kinase 1 (PGK1). Then PGK1 was produced and tested with enzyme-linked immunosorbent assay (ELISA), Western blotting, immunoprecipitation and competitive inhibition immunofluorescence assay. Immunoglobulin (Ig)G against PGK1 was detected in 46% (23 of 50) sera of KD patients, 13% (five of 38) sera in febrile non-KD patients (FC) and 2 center dot 6% (one of 38) sera in healthy donors. As an immune target, PGK1 not only helps understanding of the pathogenesis, it also has potential value in facilitating the laboratory diagnosis of KD.

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出版当年[2017]版:
大类 | 3 区 医学
小类 | 3 区 免疫学
最新[2023]版:
大类 | 3 区 医学
小类 | 3 区 免疫学
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出版当年[2016]版:
Q2 IMMUNOLOGY
最新[2023]版:
Q3 IMMUNOLOGY

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第一作者机构: [1]112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, and
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通讯机构: [1]112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, and [2]Department of Pediatrics, Beijing Children’s Hospital Affiliated to Capital Medical University, Beijing, China [*1]School of Chemistry and Biological Engineering, University of Science and Technology Beijing. 30 Xueyuan Road, Beijing 100083, P. R. China. [*2]Department of Pediatrics, Beijing Children’s Hospital, Capital Medical University, 56 Nan Li Shi Road, Beijing 100045, China
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