机构:[1]112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, and[2]Department of Pediatrics, Beijing Children’s Hospital Affiliated to Capital Medical University, Beijing, China临床科室新生儿中心首都医科大学附属北京儿童医院
Kawasaki disease (KD) is an immune-mediated vasculitis with symptoms that mimic febrile illness; the immune origin has been suggested but never proved. First, cell chip technology was used to screen immune targets cells. With the indirect immunofluorescence assay we found that the HeLa cell chip could be recognized by KD patient serum samples. The target cell proteome was extracted as antigens, and antigen recognition reaction was performed using the patients' serum as antibodies and the detected target protein was detected and identified as phosphoglycerate kinase 1 (PGK1). Then PGK1 was produced and tested with enzyme-linked immunosorbent assay (ELISA), Western blotting, immunoprecipitation and competitive inhibition immunofluorescence assay. Immunoglobulin (Ig)G against PGK1 was detected in 46% (23 of 50) sera of KD patients, 13% (five of 38) sera in febrile non-KD patients (FC) and 2 center dot 6% (one of 38) sera in healthy donors. As an immune target, PGK1 not only helps understanding of the pathogenesis, it also has potential value in facilitating the laboratory diagnosis of KD.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81571592]; National S&T Major Project of China
第一作者机构:[1]112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, and
共同第一作者:
通讯作者:
通讯机构:[1]112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, and[2]Department of Pediatrics, Beijing Children’s Hospital Affiliated to Capital Medical University, Beijing, China[*1]School of Chemistry and Biological Engineering, University of Science and Technology Beijing. 30 Xueyuan Road, Beijing 100083, P. R. China.[*2]Department of Pediatrics, Beijing Children’s Hospital, Capital Medical University, 56 Nan Li Shi Road, Beijing 100045, China
推荐引用方式(GB/T 7714):
Cui J.,Zhou Y.,Hu H.,et al.PGK1 as an immune target in Kawasaki disease[J].CLINICAL AND EXPERIMENTAL IMMUNOLOGY.2018,194(3):371-379.doi:10.1111/cei.13204.
APA:
Cui, J.,Zhou, Y.,Hu, H.,Zhao, L.,Du, Z.&Du, H..(2018).PGK1 as an immune target in Kawasaki disease.CLINICAL AND EXPERIMENTAL IMMUNOLOGY,194,(3)
MLA:
Cui, J.,et al."PGK1 as an immune target in Kawasaki disease".CLINICAL AND EXPERIMENTAL IMMUNOLOGY 194..3(2018):371-379