A resequencing pathogen microarray method for high-throughput molecular diagnosis of multiple etiologies associated with central nervous system infection
Central nervous system infection (CNSI) results in significant health and economic burdens worldwide, but the diversity of causative pathogens makes differential diagnosis very difficult. Although PCR and real-time fluorescent quantitative PCR (q-PCR) assays are widely applied for pathogen detection, they are generally optimized for the detection of a single or limited number of targets and are not suitable for the diagnosis of numerous CNSI agents. In this study, we describe the development of a resequencing pathogen microarray (RPM-IVDC4) method for the simultaneous detection of viruses, bacteria, fungi and parasites that cause CNSI. The test panel of this assay included more than 100 microorganism species across 45 genera and 30 families. The analytical specificity and sensitivity were examined using a panel of positive reference strains, and the clinical performance was evaluated using 432 clinical samples by comparing the results with q-PCR assays. Our results demonstrated good performance of the RPM-IVDC4 assay in terms of sensitivity, specificity and detection range, suggesting that the platform can be further developed for high-throughput CNSI diagnosis.
基金:
National Key Research and Development Plan [2016TFC1202700, 2016YFC120090]; Beijing Municipal Science and Technology Commission Project [D151100002115003]; Capital Clinical Feature Project of Beijing Technology Program [Z151100004015046]; Guangzhou Municipal Science and Technology Commission Project [2015B2150820]
第一作者机构:[1]Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China;
通讯作者:
通讯机构:[1]Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China;[2]Childrens Hosp Hebei Prov, Neurol Dept, Shijiazhuang, Hebei, Peoples R China;
推荐引用方式(GB/T 7714):
Wang Ji,Yu Panhui,Xie Zhengde,et al.A resequencing pathogen microarray method for high-throughput molecular diagnosis of multiple etiologies associated with central nervous system infection[J].ARCHIVES OF VIROLOGY.2017,162(12):3769-3778.doi:10.1007/s00705-017-3550-7.
APA:
Wang, Ji,Yu, Panhui,Xie, Zhengde,Yan, Tengfei,Chen, Chen...&Ma, Xuejun.(2017).A resequencing pathogen microarray method for high-throughput molecular diagnosis of multiple etiologies associated with central nervous system infection.ARCHIVES OF VIROLOGY,162,(12)
MLA:
Wang, Ji,et al."A resequencing pathogen microarray method for high-throughput molecular diagnosis of multiple etiologies associated with central nervous system infection".ARCHIVES OF VIROLOGY 162..12(2017):3769-3778