详情页 - 首都医科大学宣武医院知识库

当前位置：首页 > 详情页

Multiresistant ST59-SCCmec IV-t437 clone with strong biofilm-forming capacity was identified predominantly in MRSA isolated from Chinese children

文献详情

资源类型：

收录情况： ◇ SCIE

作者：

机构： [1]Capital Med Univ, Natl Ctr Childrens Hlth, Beijing Childrens Hosp, Pediat Intens Care Unit, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China; [2]Capital Med Univ, Beijing Pediat Res Inst,Beijing Childrens Hosp,Na, MOE Key Lab Major Dis Children,Beijing Key Lab Pe, Natl Key Discipline Pediat,Natl Clin Res Ctr Resp, Beijing 100045, Peoples R China; [3]Capital Med Univ, Natl Ctr Childrens Hlth, Bacteriol Lab, Beijing Childrens Hosp, Beijing 100045, Peoples R China

出处：

DOI：

ISSN：

关键词： Staphylococcus aureus Biofilm Antimicrobial resistance Clonal lineage Pediatrician China

摘要：

Background: This study aimed to investigate the clinical and molecular epidemiology and biofilm formation of Staphylococcus aureus (SA) isolated from pediatricians in China. Methods: SA strains were isolated from Beijing Children's hospital from February 2016 to January 2017. Isolates were typed by multilocus sequence typing (MLST), spa and SCCmec typing (for Methicillin-resistant SA [MRSA] only). Antimicrobial susceptibility testing was performed by agar dilution method except sulphamethoxazole/trimethoprim (E-test method). Biofilm formation and biofilm associated genes were detected. Results: Totally 104 children (41 females and 63 males; median age, 5.2 months) were enrolled in this study, in which 60 patients suffered from MRSA infection. Among the 104 cases, 54.8% were categorized as community associated SA (CA-SA) infections. The children under 3 years were more likely to occur CA-SA infections compared with older ones (P = 0.0131). ST59-SCCmec IV-t437 (61.7%) was the most prevalent genotype of MRSA, and ST22-t309 (18.2%), ST5-t002 (9.1%), ST6-t701 (9.1%), ST188-t189 (9.1%) were the top four genotypes of methicillin-sensitive SA (MSSA). All the present isolates were susceptible to linezolid, vancomycin, trimethoprim-sulfamethoxazole, mupirocin, tigecyclin, fusidic acid. No erythromycin-susceptible isolate was determined, and only a few isolates (3.8%) were identified as susceptible to penicillin. Multi-drug resistant isolates were reponsible for 83.8% of the ST59-SCCmec IV-t437 isolates. The isolates with strong biofilm formation were found in 85% of MRSA and 53.2% of MSSA, and in 88.7% of ST59-SCCmec IV-t437 isolates. Biofilm formation ability varied not only between MRSA and MSSA (P = 0.0053), but also greatly among different genotypes (P < 0.0001). The prevalence of the biofilm associated genes among ST59-SCCmec IV-t437 clone was: icaA (100.0%), icaD (97.3%), fnbpA (100.0%), fnbpB (0), clfA (100%), clfB (100%), cna (2.7%), bbp (0), ebpS (88.5%), sdrC (78.4%), sdrD (5.4%), and sdrE (94.5%). Conclusions: These results indicated strong homology of the MRSA stains isolated from Chinese children, which was caused by spread of multiresistant ST59-SCCmec IV-t437 clone with strong biofilm formation ability. The MSSA strains, in contrast, were very heterogeneity, half of which could produce biofilm strongly.

基金：

语种：

被引次数：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2016]版：

大类 | 3 区医学

小类 | 3 区传染病学

最新[2023]版：

大类 | 3 区医学

小类 | 3 区传染病学

JCR分区：

出版当年[2015]版：

Q2 INFECTIOUS DISEASES

最新[2023]版：

Q2 INFECTIOUS DISEASES

影响因子： 3.4 最新[2023版] 3.3 最新五年平均 2.69 出版当年[2015版] 2.864 出版当年五年平均 2.613 出版前一年[2014版] 2.768 出版后一年[2016版]

第一作者：

第一作者机构： [1]Capital Med Univ, Natl Ctr Childrens Hlth, Beijing Childrens Hosp, Pediat Intens Care Unit, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China;

通讯作者：

通讯机构： [1]Capital Med Univ, Natl Ctr Childrens Hlth, Beijing Childrens Hosp, Pediat Intens Care Unit, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China;

推荐引用方式(GB/T 7714)：

APA：

MLA：

Multiresistant ST59-SCCmec IV-t437 clone with strong biofilm-forming capacity was identified predominantly in MRSA isolated from Chinese children

文献详情

相关文献