The morphologies of alveolar rhabdomyosarcoma (ARMS) are various. Some cases entirely lack an alveolar pattern and instead display a histological pattern that overlaps with embryonal rhabdomyosarcoma (ERMS). The method of pathological diagnosis of ARMS and ERMS has been updated in the 4th edition of the World Health Organization's guidelines for classification of skeletal muscle tumors. Under the new guidelines, there is still no molecular test to distinguish between these two subtypes of rhabdomyosarcoma (RMS). In the present study, we applied fluorescent in situ hybridization (FISH) and found that the Forkhead box O1 (FOXO1) gene broke apart, amplified, and displayed an aneuploid signal that was related to the RMS pathological subtype. Aside from the fact that FOXO1 break-apart and its amplification were correlated with atypical ARMS, aneuploidies were usually found in atypical ERMS. In conclusion, our results detail a potential biomarker to improve the accuracy of pathological diagnosis by discriminating between atypical ARMS and atypical ERMS.
基金：
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [ZYLX201508]; Beijing Health System Top Level Health Technical Personnel Training Plan [20153079]; Beijing Talents Fund [2015000021469G210]
第一作者机构：[1]Capital Med Univ, Beijing Childrens Hosp, Dept Pathol, Natl Ctr Childrens Hlth, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Childrens Hosp, Dept Pathol, Natl Ctr Childrens Hlth, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Key Lab Pediat Dis Otolaryngol Head & Nec, MOE Key Lab Major Dis Children, Beijing Pediat Res Inst,Beijing Childrens Hosp,Na, Beijing 100045, Peoples R China;[3]Capital Med Univ, Biobank Clin Data & Samples Pediat, Beijing Pediat Res Inst, Beijing Childrens Hosp,Natl Ctr Childrens Hlth, Beijing 100045, Peoples R China;[4]Capital Med Univ, Beijing Childrens Hosp, Dept Otolaryngol Head & Neck Surg, Natl Ctr Childrens Hlth, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Fu Libing,Jin Yaqiong,Jia Chao,et al.Detection of FOXO1 break-apart status by fluorescence in situ hybridization in atypical alveolar rhabdomyosarcoma[J].SCIENCE CHINA-LIFE SCIENCES.2017,60(7):721-728.doi:10.1007/s11427-017-9082-9.
APA：
Fu, Libing,Jin, Yaqiong,Jia, Chao,Zhang, Jie,Tai, Jun...&He, Lejian.(2017).Detection of FOXO1 break-apart status by fluorescence in situ hybridization in atypical alveolar rhabdomyosarcoma.SCIENCE CHINA-LIFE SCIENCES,60,(7)
MLA：
Fu, Libing,et al."Detection of FOXO1 break-apart status by fluorescence in situ hybridization in atypical alveolar rhabdomyosarcoma".SCIENCE CHINA-LIFE SCIENCES 60..7(2017):721-728
