机构：[1]Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ, Beijing 100045, Peoples R China;首都医科大学附属北京儿童医院[2]Capital Med Univ, Beijing Childrens Hosp, Lab Immunol, Beijing 100045, Peoples R China;科研平台肿瘤与免疫研究室首都医科大学附属北京儿童医院[3]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Ctr Med Genet, Beijing 100045, Peoples R China;临床科室科研平台内分泌科儿科研究所首都医科大学附属北京儿童医院[4]Capital Med Univ, Beijing Childrens Hosp, Dept Pediat Endocrinol & Genet Metab, Beijing 100045, Peoples R China;临床科室新生儿中心首都医科大学附属北京儿童医院[5]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Immunol Lab, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China科研平台儿科研究所首都医科大学附属北京儿童医院
Flow cytometric analysis is important for the investigation and clinical preparation of lymphocytes from children. However, the strict requirement of cell freshness and inter-assay variability limits the application of this methodology for pediatric investigations. Therefore, it is necessary to identify a reliable cryopreservative method capable of maintaining high cell viability and proper cell function in lymphocytes from children. In the present study, eight commonly-used cell cyropreservative methods were used, and their effects on cell viability, surface marker expression and cell function were examined. In addition, how these methods affect the distribution of T-cell receptor V beta subfamilies were also determined. The results of the present study provided valuable experimental evidence, based on which the optimal method for the cryopreservation of lymphocytes from children in pediatric investigations and clinical applications can be selected.
基金：
Beijing Children's Hospital, a teaching hospital affiliated with Capital Medical University (Beijing, China)
第一作者机构：[1]Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Childrens Hosp, Lab Immunol, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Childrens Hosp, Lab Immunol, Beijing 100045, Peoples R China;[5]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Immunol Lab, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Chen Xi,Zhang Hui,Mou Wenjun,et al.Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation[J].MOLECULAR MEDICINE REPORTS.2016,14(5):4301-4308.doi:10.3892/mmr.2016.5780.
APA：
Chen, Xi,Zhang, Hui,Mou, Wenjun,Qi, Zhan,Ren, Xiaoya...&Gui, Jingang.(2016).Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation.MOLECULAR MEDICINE REPORTS,14,(5)
MLA：
Chen, Xi,et al."Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation".MOLECULAR MEDICINE REPORTS 14..5(2016):4301-4308
