Allicin, a sulphur containing compound isolated from the bulb of Allium sativum, confirm the various pharmacological activity such as antioxidant and antiinflammatory effects. The current study was intended to evaluate the effect of allicin on the IL-1 beta-Induced inflammatory cytokines in Human Osteoarthritis Chondrocytes. The chondrocytes were stimulated with IL-1 beta in the presence or absence of allicin. Moreover, the western blot analyses was used for the estimation of the nuclear factor-kappa B (NF-kappa B), c-Jun N-terminal kinase (JNK), cyclooxygenase-2 (COX-2), inhibitory kappa B (I kappa B alpha), extracellular signal-regulated kinase (ERK), iNOS and p38 expression. The level of PGE(2) and NO production in the IL-1 beta treatment were determined via using the ELISA and Griess reagent. In the current study, allicin was found to be significantly inhibited the IL-1 beta induced iNOS and COX-2 expression in dose dependent manner. On the other hand, allicin in dose-dependent manner causes significant (P<0.01) reduction of the IL-1 beta-induced PGE(2), NO and collagenase-3 (MMP-13) production. Allicin also showed significant reduction of the IL-1 beta-induced mitogen-activated protein kinase (MAPK) and NF-kappa B activation. In conclusion, allicin showed efficient attenuation of the osteoarthritis chondrocytes inflammatory response triggered by IL-1 beta. The result of the current study confirmed that allicin may be beneficial agent in the management or control of osteoarthritis.
第一作者机构：[1]Capital Med Univ, Beijing Tiantan Hosp, Dept Orthopaed, Beijing 100050, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tiantan Hosp, Dept Orthopaed, Beijing 100050, Peoples R China;[2]Capital Med Univ, Beijing Tiantan Hosp, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Lin,Pan Hai-Tao,Wang Bing,et al.Allicin reduces IL-1 beta-induced inflammatory cytokines via attenuating the NF-kappa B and MMP3 activation in human osteoarthritis chondrocytes model[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY.2016,9(10):10320-10326.
APA：
Wang, Lin,Pan, Hai-Tao,Wang, Bing,Liu, Yang,Fei, Kai&Han, Wei-Feng.(2016).Allicin reduces IL-1 beta-induced inflammatory cytokines via attenuating the NF-kappa B and MMP3 activation in human osteoarthritis chondrocytes model.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,9,(10)
MLA：
Wang, Lin,et al."Allicin reduces IL-1 beta-induced inflammatory cytokines via attenuating the NF-kappa B and MMP3 activation in human osteoarthritis chondrocytes model".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 9..10(2016):10320-10326
