Methylation of Werner syndrome protein is associated with the occurrence and development of invasive meningioma via the regulation of Myc and p53 expression
机构：[1]Capital Med Univ, Beijing Tian Tan Hosp, Dept Neurosurg, Beijing 100050, Peoples R China;[2]Capital Med Univ, Beijing Tian Tan Hosp, Dept Neurosurg, 6 Tiantan Xili, Beijing 100050, Peoples R China
The aim of the present study was to investigate the positive rate of Werner syndrome protein (WRN) methylation in meningioma patients, and further assess the association between WRN methylation and the occurrence of meningioma. A total of 56 consecutive meningioma patients and 26 healthy individuals were enrolled in the study. A methylation-specific polymerase chain reaction assay was performed to detect the positive rate of WRN methylation in the peripheral blood and tissue samples collected from the recruited subjects. In addition, western blot analysis was performed to determine the protein expression levels of WRN, Myc and p53 in the peripheral blood and tissue samples. The positive rate of WRN methylation in the peripheral blood of the meningioma group was increased when compared with the control group (P<0.05). In addition, the protein expression levels of WRN were significantly decreased in the peripheral blood and tissue samples collected from the individuals with a positive WRN methylation status (P<0.05), as compared with the samples without WRN methylation. Furthermore, the protein expression levels of Myc and p53 were increased in the peripheral blood and tissue samples that exhibited positive WRN methylation when compared with those without WRN methylation (P<0.05). Therefore, WRN methylation was demonstrated to be associated with the occurrence and development of invasive meningioma, possibly through the regulation of Myc and p53 expression.
基金：
Natural Science Foundation of ChinaNational Natural Science Foundation of China [81341059]; Beijing Nova ProgramBeijing Municipal Science & Technology Commission [2012033]
第一作者机构：[1]Capital Med Univ, Beijing Tian Tan Hosp, Dept Neurosurg, Beijing 100050, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tian Tan Hosp, Dept Neurosurg, Beijing 100050, Peoples R China;[2]Capital Med Univ, Beijing Tian Tan Hosp, Dept Neurosurg, 6 Tiantan Xili, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714)：
Li Puxian,Hao Shuyu,Bi Zhiyong,et al.Methylation of Werner syndrome protein is associated with the occurrence and development of invasive meningioma via the regulation of Myc and p53 expression[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2015,10(2):498-502.doi:10.3892/etm.2015.2519.
APA：
Li, Puxian,Hao, Shuyu,Bi, Zhiyong,Zhang, Junting,Wu, Zhen&Ren, Xiaohui.(2015).Methylation of Werner syndrome protein is associated with the occurrence and development of invasive meningioma via the regulation of Myc and p53 expression.EXPERIMENTAL AND THERAPEUTIC MEDICINE,10,(2)
MLA：
Li, Puxian,et al."Methylation of Werner syndrome protein is associated with the occurrence and development of invasive meningioma via the regulation of Myc and p53 expression".EXPERIMENTAL AND THERAPEUTIC MEDICINE 10..2(2015):498-502
