机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Nutr Res Unit, Beijing 100045, Peoples R China;科研平台儿科研究所首都医科大学附属北京儿童医院[2]Columbia Univ, Coll Phys & Surg, Inst Human Nutr, New York, NY USA;[3]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Nutr Res Unit, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China科研平台儿科研究所首都医科大学附属北京儿童医院
We report evidence of a detailed epigenetic modification of the leptin promoter and the effects of n-3 polyunsaturated fatty acids (n-3 PUFAs), which is closely associated with the leptin gene transcription in obesity. In the adipose tissue of diet induced obese (DIO) mice, methylation of the CpG island and the binding of methyl-CpG-binding domain protein 2 (MBD2) and DNA methyltransferases (DNMTs) at the leptin promoter are increased and RNA Pol II is decreased. Additionally, histones H3 and H4 are hypoacetylated, lysine 4 of histone H3 (H3K4) is hypomethylated and the binding of histone deacetylases (HDACs) 1, 2 and 6 is increased at the leptin promoter in the DIO mice. These modifications may serve a feedback role to maintain leptin concentrations within a normal range. The regulation of leptin transcriptional expression by n-3 PUFAs is mediated, at least in part, by epigenetic targets, such as MBD2 and histone modifications.
基金：
Nutricia Research Foundation (The Netherlands)Netherlands Government [2009-15, 2010-E4]; International Training Fellowship from the Nutricia Research Foundation (The Netherlands) [2011-T2]; Research Funds of Profession Quota Budget from the BeijingMunicipal Science and Technology Commission [2012-bjsekyjs-2]
第一作者机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Nutr Res Unit, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Nutr Res Unit, Beijing 100045, Peoples R China;[3]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Nutr Res Unit, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Shen Wenwen,Wang Cui,Xia Lulu,et al.Epigenetic Modification of the Leptin Promoter in Diet-Induced Obese Mice and the Effects of N-3 Polyunsaturated Fatty Acids[J].SCIENTIFIC REPORTS.2014,4:-.doi:10.1038/srep05282.
APA：
Shen, Wenwen,Wang, Cui,Xia, Lulu,Fan, Chaonan,Dong, Hua...&Qi, Kemin.(2014).Epigenetic Modification of the Leptin Promoter in Diet-Induced Obese Mice and the Effects of N-3 Polyunsaturated Fatty Acids.SCIENTIFIC REPORTS,4,
MLA：
Shen, Wenwen,et al."Epigenetic Modification of the Leptin Promoter in Diet-Induced Obese Mice and the Effects of N-3 Polyunsaturated Fatty Acids".SCIENTIFIC REPORTS 4.(2014):-
