机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Key Lab Major Dis Children, Beijing 100045, Peoples R China;科研平台儿科研究所首都医科大学附属北京儿童医院[2]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Natl Key Discipline Pediat, Beijing 100045, Peoples R China;科研平台儿科研究所首都医科大学附属北京儿童医院[3]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Key Lab Major Dis Children, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China科研平台儿科研究所首都医科大学附属北京儿童医院
In order to evaluate the diagnostic accuracy of the Xpert MTB/RIF assay on childhood pulmonary tuberculosis (PTB) using bronchoalveolar lavage fluid (BALF), we evaluated the sensitivity, specificity, positive predictive value, and negative predictive value of Xpert MTB/RIF assay using BALF in comparison with acid-fast bacilli (AFB) microscopy and Mycobacterium tuberculosis (MTB) culture for diagnosing childhood PTB using Chinese "composite clinical reference standard" (CCRS) as reference standard. Two hundred fifty-five children with suspected PTB were enrolled at Beijing Children's Hospital from September 2010 to July 2013. Compared with Chinese CCRS, the sensitivity of AFB microscopy, MTB culture, and Xpert MTB/RIF assay was 8.4%, 28.9%, and 53.0%, respectively. The specificity of three assays was all 100%. Xpert MTB/RIF assay could detect 33.9% of cases with negative MTB culture, and 48.7% of cases with negative AFB microscopy. Younger age (<3 years), absence of BCG scar, and contact with TB patient were found significantly associated with a positive result of Xpert MTB/RIF assay. In conclusion, Xpert MTB/RIF assay using BALF can assist in diagnosing childhood PTB much faster when fiberoptic bronchoscopy is necessary according to the chest radiograph.
基金：
Capital health research and development of special Grant [2011-2009-05]; National Science and Technology Major Project of China [2013ZX10003003]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81271889]; Technology Foundation for Selected Overseas Chinese Scholar of Beijing
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
无
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2012]版：
无
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Key Lab Major Dis Children, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Natl Key Discipline Pediat, Beijing 100045, Peoples R China;[3]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Key Lab Major Dis Children, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Key Lab Major Dis Children, Beijing 100045, Peoples R China;[2]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Natl Key Discipline Pediat, Beijing 100045, Peoples R China;[3]Capital Med Univ, Beijing Childrens Hosp, Beijing Pediat Res Inst, Minist Educ,Key Lab Major Dis Children, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Yin Qing-Qin,Jiao Wei-Wei,Han Rui,et al.Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid[J].BIOMED RESEARCH INTERNATIONAL.2014,2014:-.doi:10.1155/2014/310194.
APA：
Yin, Qing-Qin,Jiao, Wei-Wei,Han, Rui,Jiao, An-Xia,Sun, Lin...&Shen, A-Dong.(2014).Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid.BIOMED RESEARCH INTERNATIONAL,2014,
MLA：
Yin, Qing-Qin,et al."Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid".BIOMED RESEARCH INTERNATIONAL 2014.(2014):-
