机构：[1]Stroke Center,Beijing Anzhen Hospital, Capital Medical University, Beijing 100029,首都医科大学附属安贞医院[2]Surgery Intensive Care Unit, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029,临床科室综合外科监护室(SICU)首都医科大学附属安贞医院[3]Department of Pathology, Beijing Youan Hospital, Capital Medical University, Beijing 100054,[4]Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029,临床科室心脏外科中心首都医科大学附属安贞医院[5]Cardio-Thoracic Vascular Surgery, The 306th Hospital of PLA, Beijing 100101,[6]Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015,[7]Department of General Surgery, The Second People's Hospital of Guangdong Province, Guangzhou 510515,[8]International Mongolian Hospital, Hohhot of Inner Mongolia, Hohhot 010065,[9]Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China临床科室血管科首都医科大学附属安贞医院
The development of acute lung injury (ALI) during sepsis almost doubles the mortality rate of patients. The efficacy of current treatment strategies is low as treatment is usually initiated following the onset of symptoms. Inflammation is one of the main mechanisms of autoimmune disorders and is a common feature of sepsis. The suppression of inflammation is therefore an important mechanism for the treatment of sepsis. Sirtuin 1 (Sirt1) has been demonstrated to play a role in the regulation of inflammation. Resveratrol, a potent Sirt1 activator, exhibits anti-inflammatory properties. However, the role of resveratrol for the treatment of ALI during sepsis is not fully understood. In the present study, the anti-inflammatory role of Sirt1 in the lipopolysaccharide (LPS)-induced TC-1 cell line and its therapeutic role in ALI was investigated in a mouse model of sepsis. The upregulation of matrix metalloproteinase-9, interleukin (IL)-1 beta, IL-6 and inducible nitric oxide synthase was induced by LPS in the mouse model of sepsis and the TC-1 cell line, and resveratrol suppressed the overexpression of these proinflammatory molecules in a dose-dependent manner. Resveratrol decreased pulmonary edema in the mouse model of sepsis induced by LPS. In addition, resveratrol improved lung function and reduced pathological alterations in the mouse model of sepsis. Knockdown of Sirt1 by RNA interference resulted in an increased susceptibility of TC-1 cells to LPS stimulation and diminished the anti-inflammatory effect of resveratrol. These results demonstrated that resveratrol inhibits LPS-induced ALI and inflammation via Sirt1, and indicated that Sirt1 is an efficient target for the regulation of LPS-induced ALI and inflammation. The present study provides insights into the treatment of ALI during sepsis.
基金：
National Natural Science Foundation of China [30600524]
第一作者机构：[1]Stroke Center,Beijing Anzhen Hospital, Capital Medical University, Beijing 100029,
通讯作者：
通讯机构：[6]Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015,[9]Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China[*1]Department of Vascular Surgery, Beijing Anzhen Hospital, Capital Medical University, 2 Anzhen Road, Beijing 100029, P.R. China[*2]Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, 8 Jingshun East Street, Beijing 100015, P.R. China
推荐引用方式(GB/T 7714)：
Li Tongxun,Zhang Jinglan,Feng Jiliang,et al.Resveratrol reduces acute lung injury in a LPS-induced sepsis mouse model via activation of Sirt1[J].MOLECULAR MEDICINE REPORTS.2013,7(6):1889-1895.doi:10.3892/mmr.2013.1444.
APA：
Li, Tongxun,Zhang, Jinglan,Feng, Jiliang,Li, Qiang,Wu, Lisong...&Wu, Qinghua.(2013).Resveratrol reduces acute lung injury in a LPS-induced sepsis mouse model via activation of Sirt1.MOLECULAR MEDICINE REPORTS,7,(6)
MLA：
Li, Tongxun,et al."Resveratrol reduces acute lung injury in a LPS-induced sepsis mouse model via activation of Sirt1".MOLECULAR MEDICINE REPORTS 7..6(2013):1889-1895
