Hypermethylation of two CpG sites upstream of CASP8AP2 promoter influences gene expression and treatment outcome in childhood acute lymphoblastic leukemia
DNA hypermethylation of Caspase 8 associated protein 2 (CASP8AP2) and its role in childhood acute lymphoblastic leukemia (ALL) is unclear. We analyzed methylation status of CpG sites upstream of CASP8AP2 gene in 86 children with ALL by bisulfite sequencing and quantitative PCR. Methylation percentage of two CpG sites at positions of -1189 and -1176 was inversely correlated with mRNA expression (Spearman correlation: -0.333, P = 0.002). High methylation was associated with the existence of minimal residual disease (MRD) at day 78 (P = 0.035), The patients in high methylation group had a poor treatment outcome. The combination of methylation level and MRD at day 33 might improve current risk stratification. (C) 2013 Elsevier Ltd. All rights reserved.
基金：
Beijing Natural Science FoundationBeijing Natural Science Foundation [7112051]; Beijing Health Qualified Personnel Program [2011-3-049]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81170504]; National Science & Technology Major Project of the 12th 5-Year Plan [2011ZX09302-007-01]; National Key Technologies Research & Development Program of the 11th 5-Year Plan [2007BAI04B03]
第一作者机构：[1]Capital Med Univ, Key Lab Major Dis Children, Minist Educ, Hematol Ctr,Beijing Childrens Hosp, Beijing 100069, Peoples R China;[3]Capital Med Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100069, Peoples R China;
通讯作者：
通讯机构：[3]Capital Med Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100069, Peoples R China;[4]Capital Med Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, 10 Youan Men, Beijing 100069, Peoples R China
推荐引用方式(GB/T 7714)：
Li Zhi-Gang,Jiao Ying,Li Wei-Jing,et al.Hypermethylation of two CpG sites upstream of CASP8AP2 promoter influences gene expression and treatment outcome in childhood acute lymphoblastic leukemia[J].LEUKEMIA RESEARCH.2013,37(10):1287-1293.doi:10.1016/j.leukres.2013.07.018.
APA：
Li, Zhi-Gang,Jiao, Ying,Li, Wei-Jing,Deng, Guo-Ren,Cui, Lei...&Jia, Hong-Ti.(2013).Hypermethylation of two CpG sites upstream of CASP8AP2 promoter influences gene expression and treatment outcome in childhood acute lymphoblastic leukemia.LEUKEMIA RESEARCH,37,(10)
MLA：
Li, Zhi-Gang,et al."Hypermethylation of two CpG sites upstream of CASP8AP2 promoter influences gene expression and treatment outcome in childhood acute lymphoblastic leukemia".LEUKEMIA RESEARCH 37..10(2013):1287-1293
