Background: The aim of this study was to investigate the role of pulmonary surfactant-Associated protein B (SP-B) expression in the pathogenesis of neonatal respiratory distress syndrome (RDS) via detecting the protein and mRNA expression of SP-B. Methods: A total of 60 unrelated neonates who died of RDS were chosen as the RDS group and then subgrouped into =32 weeks group, 32~37 weeks group and =37 weeks group (n=20). Sixty neonates who died of other diseases were enrolled as controls and subdivided into 3 matched groups based on the gestational age. Western blot assay and RT-PCR were employed. Results: In the RDS group, SP-B protein expression was reduced or deficient in 8 neonates of which 6 had no SP-B protein expression. In the control group, only 1 had reduced SP-B protein expression. The reduced or deficient SP-B protein expression in 9 neonates of both groups was noted in the =37 weeks group. In the RDS group, the SP-B mRNA expression was significantly lower than that in the control group. In the =37 weeks group, SP-B mRNA expression was comparable between the RDS group and control group. In the 32~37 weeks group, the SP-B mRNA expression in the RDS group was significantly reduced when compared with the control group. In the =37 weeks group, the SP-B mRNA expression in the RDS group was dramatically lower than that in the control group. Conclusions: Alteration of SP-B expression is present at transcriptional and translational levels. Reduction of SP-B mRNA and protein expression is involved in the pathogenesis of RDS.
语种：
外文
中科院(CAS)分区：
出版当年[2012]版：
大类|4 区生物
小类|4 区生化与分子生物学
最新[2023]版：
大类|4 区医学
小类|4 区生化与分子生物学
第一作者：
第一作者机构：[1]Department of Pediatrics, Affiliated Bayi Children's Hospital, General Hospital of Beijing Military Region, Beijing 100700, China
推荐引用方式(GB/T 7714)：
Yin X,Wang Y,Xie L,et al.Expression of pulmonary surfactant-Associated protein b in neonatal respiratory distress syndrome[J].JOURNAL OF MEDICAL BIOCHEMISTRY.2013,32(2):doi:10.2478/jomb-2013-0005.
APA：
Yin, X,Wang, Y,Xie, L,Kong, X,QuWang, C...&Feng, Z.(2013).Expression of pulmonary surfactant-Associated protein b in neonatal respiratory distress syndrome.JOURNAL OF MEDICAL BIOCHEMISTRY,32,(2)
MLA：
Yin, X,et al."Expression of pulmonary surfactant-Associated protein b in neonatal respiratory distress syndrome".JOURNAL OF MEDICAL BIOCHEMISTRY 32..2(2013)
