Aims MicroRNA-21 (miR-21) expression is increased in many types of human malignancy, including glioma. Recent studies report that miR-21 regulates cell invasion by targeting RECK, however, the underlying transcriptional regulation of miR-21 in glioma cells remains elusive. Results Here, we identify a positive correlation between miR-21 expression and pathological grade in glioma tissues. We demonstrate that beta-catenin pathway regulates miR-21 expression in human umbilical vein endothelial cell and glioma cells, and that this regulation is signal transducer and activator of transcription 3 (STAT3)-dependent. Further, chromatin immunoprecipitation and luciferase reporter analysis demonstrate that miR-21 is controlled by an upstream promoter containing a conserved STAT3 binding site. Notably, knockdown of miR-21-inhibited cell invasion by increasing RECK expression and decreased tumor growth in a xenograft model. Conclusion These data provide compelling evidence that beta-catenin regulation of miR-21 via STAT3 plays a role in glioma cell invasion and proliferation and indicate that STAT3 is a potential therapeutic target for glioma intervention.
第一作者机构:[1]Tianjin Med Univ, Gen Hosp, Dept Neurosurg,Minist Educ,Tianjin Key Lab Injuri, Key Lab Posttrauma Neurorepair & Regenerat Cent N, Tianjin, Peoples R China;
通讯作者:
通讯机构:[1]Tianjin Med Univ, Gen Hosp, Dept Neurosurg,Minist Educ,Tianjin Key Lab Injuri, Key Lab Posttrauma Neurorepair & Regenerat Cent N, Tianjin, Peoples R China;[6]Tianjin Neurol Inst, Lab Neuro Oncol, 154 Anshan Rd, Tianjin 300052, Peoples R China
推荐引用方式(GB/T 7714):
Han Lei,Yue Xiao,Zhou Xuan,et al.MicroRNA-21 Expression is regulated by ss-catenin/STAT3 Pathway and Promotes Glioma Cell Invasion by Direct Targeting RECK[J].CNS NEUROSCIENCE & THERAPEUTICS.2012,18(7):573-583.doi:10.1111/j.1755-5949.2012.00344.x.
APA:
Han, Lei,Yue, Xiao,Zhou, Xuan,Lan, Feng-Ming,You, Gan...&Kang, Chun-Sheng.(2012).MicroRNA-21 Expression is regulated by ss-catenin/STAT3 Pathway and Promotes Glioma Cell Invasion by Direct Targeting RECK.CNS NEUROSCIENCE & THERAPEUTICS,18,(7)
MLA:
Han, Lei,et al."MicroRNA-21 Expression is regulated by ss-catenin/STAT3 Pathway and Promotes Glioma Cell Invasion by Direct Targeting RECK".CNS NEUROSCIENCE & THERAPEUTICS 18..7(2012):573-583