We describe a multiplex PCR assay to detect the Mycobacterium tuberculosis Beijing genotype variant B0/W148, which is considered a "successful" clone of M. tuberculosis, widespread in Russia. Specificity and sensitivity of the assay were 100% based on the analysis of a collection of 516 M. tuberculosis isolates of different genotypes and origins. This assay may be used for accurate and simple detection and surveillance of this clinically and epidemiologically important variant of M. tuberculosis.
基金:
European Commission (ORCHID project) [261378]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [11-04-91172 GFEN_a]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81071315]
第一作者机构:[1]St Petersburg Pasteur Inst, Mol Microbiol Lab, St Petersburg, Russia;
通讯作者:
通讯机构:[1]St Petersburg Pasteur Inst, Mol Microbiol Lab, St Petersburg, Russia;
推荐引用方式(GB/T 7714):
Mokrousov Igor,Narvskaya Olga,Vyazovaya Anna,et al.Russian "Successful" Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening[J].JOURNAL OF CLINICAL MICROBIOLOGY.2012,50(11):3757-3759.doi:10.1128/JCM.02001-12.
APA:
Mokrousov, Igor,Narvskaya, Olga,Vyazovaya, Anna,Otten, Tatiana,Jiao, Wei-Wei...&Vishnevsky, Boris.(2012).Russian "Successful" Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening.JOURNAL OF CLINICAL MICROBIOLOGY,50,(11)
MLA:
Mokrousov, Igor,et al."Russian "Successful" Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening".JOURNAL OF CLINICAL MICROBIOLOGY 50..11(2012):3757-3759