Reverse transcription loop-mediated isothermal amplification (RI-LAMP), which is a visual assay for nucleic acids, is performed in a single step using one tube at 65 degrees C for 1.5h. In this study, RI-LAMP was established as a method for the detection of enterovirus 71 (EV71). The detection limit of the assay was approximately 10 copies, and no cross-reactivity was noted with Coxsackievirus A16, echovirus, human rotavirus (HRV) or norovirus. This assay, which offers greater sensitivity at a lower cost compared with the conventional reverse transcription polymerase chain reaction (RT-PCR), was validated using 252 clinical specimens that had been confirmed by laboratory diagnosis using RT-PCR. Both methods produced the same results with 52 positive samples. The RT-LAMP-based assay does not require specialised equipment, and therefore, it can be performed conveniently during an outbreak or under field conditions. In brief, the RI-LAMP-based assay provided a simple, rapid and efficient method for the detection of EV71 nucleic acid under field conditions. (C) 2011 Elsevier B.V. All rights reserved.
基金：
China Mega-Project for Infectious Diseases [2009ZX10004-101, 2008ZX10004-002]
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2011]版：
大类|3 区医学
小类|4 区生化研究方法4 区生物工程与应用微生物4 区病毒学
最新[2023]版：
大类|4 区医学
小类|3 区生化研究方法3 区生物工程与应用微生物4 区病毒学
JCR分区：
出版当年[2010]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3BIOCHEMICAL RESEARCH METHODSQ3VIROLOGY
最新[2023]版：
Q3BIOCHEMICAL RESEARCH METHODSQ3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3VIROLOGY
第一作者机构：[1]Chinese Ctr Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Natl Inst Viral Dis Control & Prevent, Beijing 100052, Peoples R China;
通讯作者：
通讯机构：[1]Chinese Ctr Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Natl Inst Viral Dis Control & Prevent, Beijing 100052, Peoples R China;[4]Chinese Ctr Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Natl Inst Viral Dis Control & Prevent, Ying Xin Rd 100, Beijing 100052, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Xiang,Zhu Jun-ping,Zhang Qian,et al.Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)[J].JOURNAL OF VIROLOGICAL METHODS.2012,179(2):330-334.doi:10.1016/j.jviromet.2011.11.019.
APA：
Wang, Xiang,Zhu, Jun-ping,Zhang, Qian,Xu, Zi-gang,Zhang, Fang...&Zheng, Li-shu.(2012).Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).JOURNAL OF VIROLOGICAL METHODS,179,(2)
MLA：
Wang, Xiang,et al."Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)".JOURNAL OF VIROLOGICAL METHODS 179..2(2012):330-334
