Purpose: The aim of this study was to identify the presence of collagen-binding integrin subunits in human scleral fibroblasts (HSFs) and investigate their actual functions in maintaining the mechanical creep properties of the HSFs-seeded collagen matrix. Methods: Primary HSFs were cultured in vitro. Reverse-transcription PCR was used to detect mRNA expression of integrin alpha 1, alpha 2, and beta 1 subunits in HSFs. In addition, western blot analysis and immunofluorescence were used to detect their protein in HSFs. Monoclonal antibodies were applied directly against the extracellular domains of integrin subunits in HSFs cultured in the three-dimensional collagen gels to block the interaction between HSFs and the extracellular collagen matrix. The effects of anti-integrin antibodies on HSFs morphology in collagen gel were observed. The effects of the added antibodies on fibroblast-mediated collagen gels' contraction were evaluated. Furthermore, the changes in mechanical creep properties of collagen gel were measured by a biomechanics test instrument. Results: The mRNA and protein expressions of collagen-binding integrin alpha 1, alpha 2, and beta 1 subunits were present in HSFs. The elongated bipolar cells converted to spherical shapes after 6 h after the addition of integrin alpha 1 beta 1 and alpha 2 beta 1 antibody. The blocking of integrin alpha 1 beta 1 and alpha 2 beta 1 subunits noticeably decreased the contraction in the collagen gels. In addition, all samples were subjected to a constantly applied load of 0.03 N for 600 s. The blocking of integrin alpha 1 beta 1 and alpha 2 beta 1 subunits also induced increases in the values of final extension, creep extension, and creep rate, compared to those of the controls (p<0.01). Furthermore, the creep elements were significantly increased with the augmentation of the integrin antibody dose (p<0.01). The final extension of the integrin alpha 2 beta 1 antibody (1 mu g/ml or 4 mu g/ml) group was significantly higher compared to that of the integrin alpha 1 beta 1 antibody (1 mu g/ml or 4 mu g/ml) group (p<0.01). However, the creep extension and creep rate of the integrin alpha 2 beta 1 antibody (1 mu g/ml or 4 mu g/ml) group were not significantly different from those in the integrin alpha 1 beta 1 antibody (1 mu g/ml or 4 mu g/ml) group (p>0.05). Conclusions: Our findings suggested that HSF integrin alpha 1 beta 1 and alpha 2 beta 1 participated in maintaining the mechanical creep properties of the HSFs-seeded collagen matrix. Furthermore, integrin alpha 2 beta 1 might play a more crucial role in maintaining the mechanical creep properties of the collagen matrix than does integrin alpha 1 beta 1.