We aimed to investigate the effect of bone marrow stromal cell-conditioned medium (BCM) on glutamate uptake of peroxide (H2O2)-injured astrocytes. Bone marrow stromal cells (BMSC) were isolated from rat bone marrow. Confluent BMSC cultures were incubated with serum-free Dulbecco's Modified Eagle's Medium to create the BCM. Astrocytes were isolated from 1-day-old rats. H2O2-injured astrocytes were cultured in BCM (experimental group) or serum-free medium (control group). The labeled glutamate (H-3-L-glutamate) uptake by H2O2-injured astrocytes with or without BCM was compared after I and 3 days. We found that astrocytes Cultured in BCM exhibited increased glutamate uptake compared to those cultured in serum-free medium following H2O2-induced injury (p < 0.01) and concluded that BCM increased the glutamate uptake capability of H2O2-injured rat astrocytes. The therapeutic benefits associated with BMSC transplantation following brain injury might be partly due to increased glutamate uptake by astrocytes. (C) 2009 Elsevier Ltd. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30471766]
第一作者机构：[2]Capital Med Univ, Beijing Tiantan Hosp, Dept Neurosurg, Beijing 100050, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Neurosurg Inst, Beijing 100050, Peoples R China;[3]Capital Med Univ, Beijing Neurosurg Inst, Tiantan Xili 6, Beijing 100050, Peoples R China
推荐引用方式(GB/T 7714)：
Gui Song-bai,Zhang Ya-zhuo,Sun Mei-zhen,et al.Effect of bone marrow stromal cell-conditioned medium on the glutamate uptake of peroxide-injured astrocytes[J].JOURNAL OF CLINICAL NEUROSCIENCE.2009,16(9):1205-1210.doi:10.1016/j.jocn.2008.11.021.
APA：
Gui, Song-bai,Zhang, Ya-zhuo,Sun, Mei-zhen,Wang, Hong-yun,He, Yue&Li, Dan.(2009).Effect of bone marrow stromal cell-conditioned medium on the glutamate uptake of peroxide-injured astrocytes.JOURNAL OF CLINICAL NEUROSCIENCE,16,(9)
MLA：
Gui, Song-bai,et al."Effect of bone marrow stromal cell-conditioned medium on the glutamate uptake of peroxide-injured astrocytes".JOURNAL OF CLINICAL NEUROSCIENCE 16..9(2009):1205-1210
