Objective: To evaluate the feasibility of microwave ablation (MA) for canine lung tissue, and to provide experimental evidences for clinical application. Methods: Pulmonary tissues of 15 domesticated canine were ablated with different microwave output power (20 W, 40 W, 60 W). Ablation lesions were excised within 1 h after MA and pathological examine was performed. Results: (1)One canine died during MA because of heart injury, the others tolerated the procedure well. (2)The areas of ablation was different according to different power. The ablative area of 40 W was the largest, the mean diameter was (1.72±0.13) cm, the mean area was (3.12±0.08) cm 2, while the ablative area of 20 W was the second large, with the mean diameter of (1.07±0.05) cm, the mean area of (2.24±0.31) cm 2, and the ablative area of 60 W was the smallest ([1.14±0.07] cm 2), with diameter of (1.19±0.23) cm. The shape of the ablative lesion was ellipse near global. (3)From the center to peripheral area, the pathologic changes were characterized as carbonization, coagulation necrosis, and peripheral reactive band. Conclusion: Microwave ablation for canine lung tissue is minimally invasive and feasible, and 40 W is suitable output power producing ablative area of (3.12±0.08) cm 2 in the intravital canine lung tissue.