The aim of this study was to develop a sensitive and reliable method for the molecular identification of pathogenic bacteria. A multiplex PCR-based reverse line blot (mPCR/RLB) hybridisation assay was developed and evaluated for the rapid identification of 24 systemic and respiratory bacterial pathogens in routine diagnosis. All species-specific probes designed for the RLB hybridised with amplified DNA only from the corresponding species. Sensitivity limits of the mPCR/RLB assay varied among the 24 target organisms from 0.05 pg to 0.5 ng of genomic DNA. The sensitivity of the assay was 2 x 10(2) CFU/mL for Streptococcus pneumoniae and 6 x 10(2) CFU/mL for Escherichia coli. The specificity of each probe was tested against 24 species. There were no cross-reactions among any of the 43 probes. The mPCR/RLB assay appeared to be a useful alternative tool for the molecular identification of common pathogens.
第一作者机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing 100045, Peoples R China;
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Childrens Hosp, Beijing 100045, Peoples R China;[3]Fudan Univ, Childrens Hosp, Shanghai, Peoples R China;[4]Capital Med Univ, Beijing Childrens Hosp, Nan Lishi Rd 56, Beijing 100045, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Y.,Kong F.,Gilbert G. L.,et al.Use of a multiplex PCR-based reverse line blot (mPCR/RLB) hybridisation assay for the rapid identification of bacterial pathogens[J].CLINICAL MICROBIOLOGY AND INFECTION.2008,14(2):155-160.doi:10.1111/j.1469-0691.2007.01890.x.
APA：
Wang, Y.,Kong, F.,Gilbert, G. L.,Brown, M.,Gao, W....&Yang, Y..(2008).Use of a multiplex PCR-based reverse line blot (mPCR/RLB) hybridisation assay for the rapid identification of bacterial pathogens.CLINICAL MICROBIOLOGY AND INFECTION,14,(2)
MLA：
Wang, Y.,et al."Use of a multiplex PCR-based reverse line blot (mPCR/RLB) hybridisation assay for the rapid identification of bacterial pathogens".CLINICAL MICROBIOLOGY AND INFECTION 14..2(2008):155-160
医学