Streptococcus agalactiae (group B streptococcus, GBS) is an important cause of sepsis in neonates and their mothers, and the elderly and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution is needed to guide the development and assess the feasibility of GBS conjugate vaccines. The authors previously developed a molecular serotype identification method based on serotype-specific PCR and partial sequencing of cps genes. In this study, a novel 10-primer pair multiplex PCR and reverse line blot (mPCR/RLB) hybridization assay was developed for simultaneous detection and serotype identification of all nine GBS serotypes. For all 316 GIBS isolates tested the mPCR/RLB results corresponded with those of conventional serotyping and individual serotype-specific PCR, and the method was more convenient and practical than either alternative.
第一作者机构：[1]ICPMR, CIDM, Westmead, NSW, Australia;[2]Capital Univ Med Sci, Beijing Childrens Hosp, Dept Dermatol, Beijing, Peoples R China
通讯作者：
通讯机构：[1]ICPMR, CIDM, Westmead, NSW, Australia;[2]Capital Univ Med Sci, Beijing Childrens Hosp, Dept Dermatol, Beijing, Peoples R China
推荐引用方式(GB/T 7714)：
Kong F,Ma L,Gilbert GL.Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization[J].JOURNAL OF MEDICAL MICROBIOLOGY.2005,54(12):1133-1138.doi:10.1099/jmm.0.46244-0.
APA：
Kong, F,Ma, L&Gilbert, GL.(2005).Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization.JOURNAL OF MEDICAL MICROBIOLOGY,54,(12)
MLA：
Kong, F,et al."Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization".JOURNAL OF MEDICAL MICROBIOLOGY 54..12(2005):1133-1138
