Objective To explore the factors which induce differentiation of embryonic neural stem cells. Methods Rat embryonic neural stem cells were co-cultured with newborn rat Schwann cells in serum-free medium. The phenotype and specific-markers including tubulin-β, glial fibrillary acidic protein (GFAP) and galactorcerebroside (GalC), were domonstrated by phase contrast microscopy and double immunofluorescence staining. Results Overall, 80%±5% of neural stem cells protruded several elongated processes and expressed tubulin-β antigen at high levels, while 20±3% of them protruded several short processes and were GalC or GFAP positive. Conclusion The factors secreted by Schwann cells could induce rat embryonic neural stem cell to differentiate.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2002]版：
无
最新[2023]版：
大类|3 区医学
小类|3 区医学：内科
第一作者：
第一作者机构：[1]Beijing Neurosurgical Institute, Beijing 100050, China. wanhong50@hotmail.com
推荐引用方式(GB/T 7714)：
万虹,安沂华,张泽舜,et al.Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells[J].CHINESE MEDICAL JOURNAL.2003,116(3):428-431.
APA：
万虹,安沂华,张泽舜,张亚卓&王忠诚.(2003).Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells.CHINESE MEDICAL JOURNAL,116,(3)
MLA：
万虹,et al."Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells".CHINESE MEDICAL JOURNAL 116..3(2003):428-431
