机构：[a]Institute of Experimental Medicine, Saint Petersburg, 197 376, Russian Federation[b]Beijing Children's Hospital, Cap. University of Medical Sciences, Beijing, 100 045, China首都医科大学附属北京儿童医院[c]University of Veterinary Medicine, Košice, 04181, Slovakia
The presence of insertion elements (IS) IS861 and IS1548 in the collection of 211 Streptococcus agalactiae strains isolated from pregnant women and dairy cows was assayed. IS861 was found in 67 human strains (59%) and 36 bovine strains (37%), IS1548 in 13 human strains (12%) and 16 bovine strains (16%). Two combinations, IS861+ IS1548- and IS861 -IS1548-, were widely distributed in both human and bovine strains. The copy number and the restriction fragment length polymorphism (RFLP) of the two IS were determined in human group B streptococcus (GBS) strains. A minimum of 8 copies of IS1548 were detected in GBS strains while the copy number of IS861 varied from 1 to 9. The number of different hybridizing patterns with IS861 and IS1548 probes was 9 and 6, respectively. These hybridization patterns were divided into several clusters. All strains with IS were also clustered according to pulsed fieldgel electrophoresis (PFGE) patterns. A correlation was found between the results of PFGE- and IS-based clustering.
基金：
We thank Prof. A.A.Totolian for valuable comments. This study was supported by funding of the Ministry of Science and Technology (P.R.China), by the Russian Foundation for Basic Research grants 00-04-49360a and 02-04-06927, and by the Slovak Grant Agency VEGA grant 1-8021-01.
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大类|4 区生物学
小类|4 区生物工程与应用微生物4 区微生物学
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推荐引用方式(GB/T 7714)：
Dmitriev A,Yang M,Shakleina E,et al.The presence of insertion elements IS861 and IS1548 in group B streptococci[J].FOLIA MICROBIOLOGICA.2003,48(1):doi:10.1007/BF02931285.
APA：
Dmitriev, A,Yang, M,Shakleina, E,Tkáčiková, E,Suvorov, A...&Yang, Y.H.(2003).The presence of insertion elements IS861 and IS1548 in group B streptococci.FOLIA MICROBIOLOGICA,48,(1)
MLA：
Dmitriev, A,et al."The presence of insertion elements IS861 and IS1548 in group B streptococci".FOLIA MICROBIOLOGICA 48..1(2003)
