Objective To investigate the mechanism of multidrug resistance (MDR) in a human glioma cell and methods for overcoming multi-drug resistance.Methods MDR cell line C6/adr was established. The expression of the mdr-1 gene and its P-glycoprotein (P-gp) in the C6/adr cell line was observed by RT-PCR and flow cytometry. The reversal of MDR by verapamil, erythromycin, dihydropyridine, P-gp monoclonal antibody and Salvia miltiorrhiza (SM) was studied by microtiter tetrazolium (MTT) assay or by high performance liquid chromatographic assay.Results The mdr-1 gene of the C6/adr cell line was positive, over-expressing P-gp. The drug-resistance of the C6/adr cell lines could be partly reversed by 2-6?μg/ml of verapamil, 50-100?μg/ml of erythromycin, or 5?μg/ml of dihydropyridine. As concentration increased, they had a better effect. Among these drugs, 100?μg/ml of erythromycin had the best result of reversal. Dihydropyridine 1?μg/ml, P-gp monoclonal antibody and SM had no effect.Conclusion The mdr-1 gene and its expression might be associated with the MDR of glioma cells. Verapamil, erythromycin and dihydropyridine could reverse the MDR of glioma cells.
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外文
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无
最新[2023]版：
大类|3 区医学
小类|3 区医学：内科
第一作者：
第一作者机构：[1]Beijing Neurosurgical Institute, Beijing 100050, China. liufuchengs@hotmail.com
推荐引用方式(GB/T 7714)：
刘福生,王忠诚,张庆林,et al.胶质瘤多药耐药细胞系C 6/adr的建立及其耐药性逆转的研究[J].CHINESE MEDICAL JOURNAL.2002,115(2):238-241.doi:10.3760/j.issn:0366-6999.2002.02.019.
APA：
刘福生,王忠诚,张庆林,历俊华&张亚卓.(2002).胶质瘤多药耐药细胞系C 6/adr的建立及其耐药性逆转的研究.CHINESE MEDICAL JOURNAL,115,(2)
MLA：
刘福生,et al."胶质瘤多药耐药细胞系C 6/adr的建立及其耐药性逆转的研究".CHINESE MEDICAL JOURNAL 115..2(2002):238-241
